家蝇介导的水稻基因逃逸风险评估(英文)

【目的】对转基因作物进行生态风险评估是大面积种植前的一个必要步骤，水稻Oryza sativa访花昆虫有上百种，包括家蝇Musca domestica。本研究旨在明确访花昆虫家蝇介导转基因水稻外源基因逃逸的风险。【方法】2010年，我们使用转基因水稻B1， B6和G8-7作为父本(花粉供体)，用同源非转基因水稻Jiazao 935和Wuyunjing 7作为母本（花粉受体），并用家蝇作为授粉昆虫，在浙江大学华家池和长兴试验基地开展了田间种植试验，对收割的后代水稻种子进行室内种植培养，对种苗用潮霉素B和草甘膦处理进行转基因杂交种检测，对存活植株进行潮霉素和草甘膦抗性基因PCR检测，测试家蝇介导的转基因水稻外源基因逃逸频率。【结果】对浙江两个测试基地3个转基因水稻品种共计超过216 500粒后代水稻种子进行的检测及结果表明，在毗邻区域杂交种少，家蝇授粉区和无家蝇授粉区转基因水稻外源基因向非转基因水稻逃逸频率均较低(0~0.64%)。【结论】家蝇介导的转基因水稻外源基因逃逸频率较低，家蝇没有增加转基因水稻外源基因逃逸的风险。

Risk assessment of gene flow in rice mediatedby the housefly Musca domestica(In English)

【Aim】 Ecological risk assessment of transgenic crops is a necessary step beforelarge-scale planting. There are hundreds of species of flower visiting insects in ricem(Oryza sativa), including the housefly (Musca domestica). This study aims to assess thepotential risk of rice gene flow mediated by flower visiting M. domestica. 【Methods】 Weconducted field experiments with the genetically modified (GM) rice lines B1, B6 and G8-7as the pollen donors, the parental non-GM rice lines Jiazao 935 and Wuyunjing 7 as thepollen recipients, and M. domestica as the pollinator in Huajiachi and Changxing testingbase of Zhejiang University in 2010. All harvested rice offspring seeds were planted in thelaboratory and the plants were used for the detection of transgenic hybrids by thehygromycin B and glyphosate treatments, and then the surviving plants were further detectedfor hygromycin- and glyphosate-resistant genes by PCR to test the gene flow of GM ricemediated by M. domestica. 【Results】 The results based on the examination of over 216 500geminated seeds from the three GM rice lines in the two testing bases in Zhejiang showedthat there were few hybrids, indicating low frequencies (0-0.64%) of transgene flow fromGM to non-GM rice at close spacing both in plots with and without M. domestica.【Conclusion】 It is concluded that the frequency of transgene flow from GM to non-GM ricemediated by M. domestica is low and M. domestica does not increase the risk of gene flow ofrice.