褐飞虱对五种杀虫剂抗性的快速检测技术

【目的】建立褐飞虱Nilaparvata lugens对常用杀虫剂抗性的快速检测技术，实时掌握田间褐飞虱种群的抗药性水平，以指导褐飞虱防控合理用药。【方法】基于玻璃瓶药膜法，研制褐飞虱3龄若虫对吡虫啉、啶虫脒、醚菊酯、毒死蜱和异丙威5种杀虫剂抗性的快速检测试剂盒；利用试剂盒测定的死亡率与稻苗浸渍法测得的抗性倍数进行相关性分析，并验证利用试剂盒快速测定褐飞虱田间种群对5种杀虫剂 抗性水平的准确性。【结果】处理1 h时吡虫啉、啶虫脒、醚菊酯、毒死蜱和异丙威对褐飞虱室内敏感种群3龄若虫的LD₉₀分别为：30.96, 92.05, 117.24, 514.21和1.24 ng/cm²。在吡虫啉、啶虫脒、醚菊酯、毒死蜱和异丙威相应诊断剂量下，贵州省不同田间种群褐飞虱3龄若虫的校正死亡率分别在23.75%~78.75%, 25.00%~78.75%, 43.75%~88.75%, 36.25%~85.00%和18.75%~67.50%之间。相关性分析表明，上述田间种群褐飞虱3龄若虫的死亡率与稻苗浸渍法测定的抗性倍数呈显著负相关，相关系数在0.8751~0.9754之间。通过快速检测试剂盒获得的死亡率及相关性方程计算得到贵州安龙地区(AL)褐飞虱种群对吡虫啉、啶虫脒、醚菊酯、毒死蜱和异丙威的推测抗性倍数分别为7.23, 3.68, 4.14, 4.12和31.18，采用稻苗浸渍法测得上述5种杀虫剂的实测抗性倍数分别为6.33, 5.24, 3.71, 4.50和26.56，表明推测抗性倍数与实测的抗性倍数结果表现一致。【结论】该快速检测试剂盒可以通过测定褐飞虱田间种群的死亡率，快速评估褐飞虱田间种群对杀虫剂的抗性水平。

Rapid detection technology for the resistance to five insecticides in the brownplanthopper,Nilaparvata lugens(Hemiptera: Delphacidae)

【Aim】 This study aims to develop a rapid detection technology for theresistance of the brown planthopper, Nilaparvata lugens, to commonly used insecticides, andto master the resistance level of its field populations in real time, so as to guide theselection and use of pesticides for control of N. lugens. 【Methods】 Based on the glassvial bioassay, a rapid detection kit for the resistance of the 3rd instar nymphs of N.lugens to five insecticides, imidacloprid, acetamiprid, ethofenprox, chlorpyrifos andisoprocarb, was developed. Correlation analysis between the mortality rate measured by therapid detection kit and the resistance ratio measured by the rice seedling dipping methodwas conducted. Additionally, the accuracy of the resistance levels of the field populationsof N. lugens to the five insecticides using the detection kit was verified. 【Results】 TheLD₉₀ values of imidacloprid, acetamiprid, etofenprox, chlorpyrifos and isoprocarb to the3rd instar nymphs of the laboratory susceptible strain of N. lugens at 1 h after treatmentwere 30.96, 92.05, 117.24, 514.21 and 1.24 ng/cm², respectively. Exposure to imidacloprid,acetamiprid, etofenprox, chlorpyrifos and isoprocarb at the diagnostic doses, the correctedmortality rates of the 3rd instar nymphs of the field populations of N. lugens in Guizhouprovince were 23.75%-78.75%, 25.00%-78.75%, 43.75%-88.75%, 36.25%-85.00% and 18.75%-67.50%, respectively. The correlation analysis showed that the mortality rate of the 3rdinstar nymphs of the above field populations of N. lugens was negatively correlated withthe resistance ratio determined by the rice seedling dipping method, and the correlationcoefficient ranged from 0.8751 to 0.9754. Meanwhile, the inferred resistance ratios of N.lugens populations from Anlong (AL) region of Guizhou Province to imidacloprid,acetamiprid, etofenprox, chlorpyrifos and isoprocarb calculated by the correlationequations using the detection kit were 7.23, 3.68, 4.14, 4.12 and 31.18, respectively,and the actual resistance ratios of the above five insecticides detected by the riceseedling dip method were 6.33, 5.24, 3.71, 4.50 and 26.56, respectively, indicatingthat the inferred resistance ratios are consistent with the actual resistance ratios.【Conclusion】 This rapid detection kit can quickly assess the insecticide resistance level of the field population of N. lugens by measuring the mortality rate of the fieldpopulation.