谢氏宽漠王HSP70基因cDNA片段的克隆及热激条件下的表达

谢氏宽漠王Mantichorula semenowi Reitter是一种沙漠指示性甲虫。本研究由该种甲虫体内克隆到两种不同的HSP70基因片段，分别为MsHSP70和MsHSC70。同源性发现表明这两个基因片段与已报道的其他昆虫的热休克蛋白核苷酸序列高度同源。半定量RT-PCR分析显示：经42℃热激1 h 后立即诱导MsHSP70表达至最高峰；在恢复到室温的1～4 h 内MsHSP70表达量逐渐降低，但仍然高于未热激对照组。而MsHSC70在42℃热激1 h后表达受到抑制，但在恢复2 h和4 h时有少量的表达，分别仅为未热激对照组的0.25和0.28倍。结果提示MsHSP70和MsHSC70在保护细胞方面具有不同的作用。本实验结果为谢氏宽漠王在极端的沙漠环境胁迫下的抗逆适应性研究提供了理论基础。

Cloning of HSP70 genes in a desert beetle Mantichorula semenowi Reitter (Coleoptera: Tenebrionidae) and their expression under heat shock

Two different HSP70 gene fragments were cloned and sequenced from a desert beetle Mantichorula semenowi Reitter in Ningxia, which were named MsHSP70 and MsHSC70 respectively. Both of them share high identity with the reported heat shock proteins of other insects. Semi-quantitative RT-PCR analysis showed that 1 h heat shock treatment at 42℃ caused rapid increase of MsHSP70 mRNA, which reached maximum levels at the end of the heat shock treatment, and decreased gradually after being moved to room temperature. Expression of MsHSC70,however, was inhibited after heat shock treatment: after recovering 2 h and 4 h, the expression levels were only 0.25 and 0.28 times that of the control, respectively. The results suggest that MsHSP70 and MsHSC70 play different roles in cell protection. The present results provide new insights into adaption mechanisms of M. semenowi to extreme environment stress.