转基因抗虫棉对棉铃虫及其内寄生蜂的双重效应

以含1%转基因（Cry1A+CpTI）抗虫棉“中抗310”棉叶粉的人工饲料为基础，建立一套抗虫棉 棉铃虫Helicoverpa armigera 棉铃虫幼虫内寄生蜂中红侧沟茧蜂Microplitis mediator和棉铃虫齿唇姬蜂Campoletis chlorideae的三级营养关系的研究系统，研究了转基因抗虫棉对棉铃虫及内寄生蜂的双重效应，分析比较了6种状态的棉铃虫生长发育动态，以及寄生蜂的生长状况。结果表明，无论是否被寄生，抗虫棉对棉铃虫生长发育的抑制作用都非常显著；寄生取食抗虫棉饲料的棉铃虫的寄生蜂，其出茧率和茧重都显著下降，对于中红侧沟茧蜂，出茧率和茧重分别下降了26.1%和1.0 mg；对于棉铃虫齿唇姬蜂，分别下降了17.9%和5.1 mg。解剖寄主发现，两种寄生蜂在取食抗虫棉饲料的寄主体内发育缓慢并出现部分畸形幼蜂。棉铃虫幼虫血淋巴总蛋白含量和血淋巴蛋白SDS-PAGE电泳分析表明，取食抗虫棉饲料后，棉铃虫血淋巴总蛋白含量低于相应的对照，推测寄主血淋巴蛋白含量降低是导致寄生蜂生长缓慢、发育不正常的一个重要原因。

Impact of transgenic Cry1A plus CpTI cotton on Helicoverpa armigera(Lepidoptera:Noctuidae) and its two endoparasitoid wasps Microplitis mediator(Hymenoptera:Braconidae) and Campoletis chlorideae (Hymenoptera:Ichneumonidae)

By concocting a cotton bollworm artificial diet containing 1% leaf powder of transgenic Cry1A plus CpTI cotton “Zhongkang 310” as cotton bollworm diet, a system with a triple nutrition relation consisting of transgenic cotton Helicoverpa rmigera two endoparasitoid wasps Microplitis mediator and Campoletis chlorideae was constructed. Six treatments of H. armigera worms were conducted for evaluating the impact of the transgenic cotton on the worm and its parasitoid wasps: (1) fed on non transgenic cotton; (2) fed on transgenic cotton; (3) fed on transgenic cotton, parasitized byM. mediator; (4) fed on non transgenic cotton, parasitized by M. mediator; (5) fed on transgenic cotton, parasitized by C. chlorideae; and (6) fed on non transgenic cotton, parasitized by C. chlorideae. Tracing growth and development of the worms and their parasitoid wasps showed that the transgenic cotton suppressed the growth and development of the worm significantly no matter whether it was parasitized by the wasps or not. The cocooning rate and cocoon weight of the two wasps parasitizing the worms fed on transgenic cotton declined greatly. For M. mediator, the cocooning rate and cocoon weight decreased by 26.1% and 1 mg respectively; and for C. chlorideae, 17.9% and 5.1 mg respectively. The larvae of the two wasps developing in the haemocoele of the worms fed n transgenic cotton grew tardily and some of them showed abnormal observed by dissection. Analysis of hemolymph protein of the worm showed that the total hemolymph protein content of the worm fed on transgenic cotton was lower than that of the control. The results implied that low host hemolymph protein content might lead to the two wasps larvae growing tardily and developing abnormally.