马尾松毛虫蛋白质、核酸酶和羧酸酯酶与耐药性的关系

马尾松毛虫(Dendrolimus Punctatus Walder)幼虫蛋白质含量，羧酸酮酶和多酚氧化酶活力与虫龄大小成正相关；氰戊菊酯处理后，兴奋期的蛋白质含量和羧酸酯酶活力上升，到抑制期均降低到正常虫体的水平，而多酚氧化酶的变动不大。正常虫体中脱氧核糖核酸酶(Dnase)和核糖核酸酶(Rnase)的活力存在差异：Dnase从3-5龄幼虫随虫体增大而上升，到6龄时明显降低；Rnase与虫龄大小成负相关；氰戊菊酯处理后，Dnase便开始下降并低于正常虫体的水平，而Rnase在兴奋期上升，到抑制期下降亦低于正常虫体水平。结果说明，除多酚氧化酶外，蛋白质、核酸酶和羧酸酯酶均与耐药性存在一定的相关性，研制酶的抑制剂具有实用意义。根据毒力测定结果，马尾松毛虫幼虫随虫龄增大而耐药力增加，氰戊菊酯对5龄幼虫是3龄的2.8倍，6龄是4龄的2.3倍。因此，掌握在4龄前进行药物防治是合理的。

CORRELATION OF PROTEIN, NUCLEASE AND CARBOXYLESTERASEWITH PESTICIDE RESISTANCE IN MASSONPINE CATERPILLAR

The content of protein and activities of carboxylesterase (CarE) and polyphenol oxidase in larval stage of the massonpine caterpillar Dendrolimus pinctatus Walker showed positive correlation with age increase in instars. After treatment with fenvalerate, the content of protein and activity of Care increased during exciting stage but reduced to normal level during inhibitory stage. The activity of polyphenol oxidase showed less change. The activity of Dnase increased with age from third to fifth instar, but decreased in the sixth instar. The activity of Rnase showed negative correlation with the increase of larval instars. After applying fenvalerate, theactivity of Dnase began to drop and that of Rnase rose during exciting stage, but dropped to normal level during inhibitory stage. The results showed correlations among protein, nuclease and Care with insecticide tolerance, except polyphenol oxidase. Based on the results of toxicity test, it is clear that the insecticide tolerance of massonpine caterpillat increased with larval instars. The lethal dosage of fenvalerate to larvae in fifth instar is 2.8 times That to larvae in third instar and that to larvae in sixth instar is 2.3 times that to larvae in fourth instar. It will be rational to carry out chemical control before larvae in fourth instar.