沃尔巴克氏体在中国三种稻飞虱中的感染

用PCR方法检测了采集于不同地域稻田的3种稻飞虱共生菌沃尔巴克氏体(Wolbachia)的感染，发现灰飞虱Laodelphax striatellus、褐飞虱Nilaparvata lugens、白背飞虱Sogatella furcifera为沃尔巴克氏体所感染。克隆了编码沃尔巴克氏体外膜蛋白质的wsp基因并进行了序列测定。对wsp的RFLP分析证实了这些飞虱为单一沃尔巴克氏体感染。研究了灰飞虱中沃尔巴克氏体所诱导的胞质不相容性及其在不同地域灰飞虱中的分布。还发现能寄生于上述3种飞虱的稻虱红螯蜂也受同种沃尔巴克氏体感染。沃尔巴克氏体可能通过这种寄生蜂在不同昆虫间横向传播。

Infection of Wolbachia in three planthopper species in China

The infection of the bacterial symbionts Wolbachia in 3 planthoppers was detected by PCR method. Laodelphax striatellus, Nilaparvata lugens and Sogatella furcifera were found to be infected with Wolbachia. The gene wsp, encoding the outer membrane protein of the symbiotic bacteria, was cloned and sequenced. The RFLP analysis confirmed that those planthoppers were infected with the single Wolbachia strain. The cytoplasmic incompatibility (CI) induced by Wolbachia in Laodelphax striatellus and the distribution of Wolbachia in this insect from different regions were studied. A parasitic wasp, Haplogonatopus apicalis, which can parasitize the planthoppers, was found to be infected with the same Wolbachia. Wolbachia might transfer between different insect horizontally via this wasp.