家蚕对浓核病毒中国株(BmDNV-3)抗性及感性品系中肠的差异蛋白质分析

【目的】通过比较家蚕Bombyx mori抗性及感性品系的中肠蛋白质表达谱，获得家蚕对家蚕浓核病毒中国株（BmDNV-3）抗性相关的蛋白。【方法】利用双向电泳(2-DE)对感性品种华八35和抗性品种秋丰接种病毒后48 h的蛋白质表达谱进行比较分析，并对其中的差异蛋白进行MALDI-TOF-TOF质谱分析，通过NCBInr和MSDB数据库进行蛋白点的鉴定和功能分析。【结果】获得重复性较好的差异蛋白点16个，其中质谱鉴定出5种蛋白，它们分别是糖基转移酶（glycosyltransferase）、糖基转移酶-S（GlcAT-S）、21.5 kD小热休克蛋白（21.5 kD small heat shock protein）、V-ATP酶（vacuolar ATP synthase）和精氨酸激酶（arginine kinase）。这5种蛋白在抗性品系秋丰中的表达量均高于感性品系华八35。【结论】糖基转移酶和糖基转移酶-S仅在抗性品系中存在，提示它们可能是与抗性有关的蛋白。此外，增强的应激反应和能量代谢也可能与家蚕对BmDNV-3的抗性产生相关。

Differential expression of midgut protein in the resistant and susceptible strains of silkworm to Bombyx mori densonucleosis virus (China isolate BmDNV-3)

Objective] Obtaining the proteins related with silkworm's resistance to Bombyx mori densonucleosis virus by comparing the 2-DE maps of proteins from midguts of the susceptible and resistant strains of silkworm.Methods] Two-dimensional electrophoresis(2-DE)was used to study the protein changes of the midgut between the non-susceptible strain(Qiufeng)and the susceptible one(Huaba35)after they were inoculated for 48 hours.The changed proteins were identified by MALDI-TOF-TOF MS.The data were analyzed in NCBInr and MSDB.Results] 16 differential proteins were obtained,of which 5 proteins including glycosyltransferase,GlcAT-S,21.5 kD small heat shock protein,vacuolar ATP synthase and arginine kinase were identified by mass spectrometry.The expression levels of the 5 proteins were higher in Qiufeng strain than in Huaba35.Conclusions] Glycosyltransferase and GlcAT-S only appeared in the resistent strain,suggesting that they might be related to the resistance of silkworm to the virus.In addition,the increased stress reaction and energy metabolism may also contribute to the Qiufeng's resistance to BmDNV-3.