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桃蚜不同蚜型DNA多态性的RAPD研究
引用本文:张素方,程家安,杨效文.桃蚜不同蚜型DNA多态性的RAPD研究[J].昆虫学报,2002,45(6):764-769.
作者姓名:张素方  程家安  杨效文
作者单位:1. 浙江大学应用昆虫研究所,杭州,310029
2. 河南农业大学植物保护系,郑州,450002
摘    要:采用RAPD方法,对全周期桃蚜的有翅产雌性母蚜、无翅性母蚜、雄蚜、卵、干母、干雌、有翅迁移蚜等蚜型的DNA遗传多态性进行了分析。结果表明:卵的DNA多态性最大,性蚜次之,孤雌生殖蚜最小;卵与其它蚜型之间在遗传上具最大差异,其中与孤雌生蚜的差异大于与性蚜的;干母、干雌和迁移蚜之间的遗传关系最近。

关 键 词:桃蚜  蚜型  RAPD  DNA多态性  聚类分析  
文章编号:0454-6296(2002)06-0764-06

RAPD analysis of different forms of the green peach aphid
ZHANG Su Fang ,CHENG Jia An ,YANG Xiao\|Wen.RAPD analysis of different forms of the green peach aphid[J].Acta Entomologica Sinica,2002,45(6):764-769.
Authors:ZHANG Su Fang  CHENG Jia An  YANG Xiao\|Wen
Institution:ZHANG Su Fang 1,CHENG Jia An 1*,YANG Xiao\|Wen 2
Abstract:The green peach aphid, Myzus persicae (Sulzer), is an important pest of agricultural and horticultural crops. Its complex life cycle and genetics are the main factors influencing population outbreaks. The RAPD technique with 7 random primers was used to analyze DNA polymorphism in seven forms of M. persicae , which were: the mother of mating forms (alate), mating females (apterous), males (alate), eggs, the fundatrix (apterous), virginoparae (apterous) and virginoparae (alate). The results showed that eggs had the largest genetic variation, followed by the sexual forms with the parthenogenetic forms having the least amount of variation. There was a close genetic relationship between the fundatrix (apterous), virginoparae (apterous) and virginoparae (alate) in the green peach aphid.
Keywords:Myzus persicae  forms  RAPD  DNA polymorphism  cluster analysis
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