| YAC介导的天蚕丝素基因向家蚕的转移
——天蚕丝素基因—YAC克隆在家蚕的表达 |
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| 引用本文： | 李振刚,范久戈.YAC介导的天蚕丝素基因向家蚕的转移
——天蚕丝素基因—YAC克隆在家蚕的表达[J].昆虫学报,1997,40(1):1-6. |
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| 作者姓名： | 李振刚 范久戈 |
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| 作者单位： | [1]中国科学技术大学生物系 [2]安徽省农业科学院蚕桑研究所 |
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| 摘 要： | 本文为天蚕Antheraea yamamai丝素基因在家蚕Bombyx mori成功表达的首次报道。
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| 关 键 词： | 家蚕 天蚕 丝素基因 酵母人工染色体 |
YAC-BASED TRANSFER OF FIBROIN GENE FROM ANTHERAEA YAMAMAI TO BOMBYX MORI--EXPRESSION OF THE YAC CLONE CONTAINING A. YAMAMA/FIBROIN GENE IN B. MORI |
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| Li Zhengang,Zhou Congzhao,Tang Hengli.YAC-BASED TRANSFER OF FIBROIN GENE FROM ANTHERAEA YAMAMAI TO BOMBYX MORI--EXPRESSION OF THE YAC CLONE CONTAINING A. YAMAMA/FIBROIN GENE IN B. MORI[J].Acta Entomologica Sinica,1997,40(1):1-6. |
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| Authors: | Li Zhengang Zhou Congzhao Tang Hengli |
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| Abstract: | This paper is the first report of the expression of Antheraea yamamai fibroingene in Bombyx mori. We have constructed an yeast artificial chromosome (YAC) clonecontaining the fibroin gene of A. yamamai, and transferred this DNA clone into the fertilized eggs of B. moti. Dot blot indicates that the fibroin gene has integrated into the genomeof B. moti. By analyzing the fibroin amino acid composition and the silk solubility, wefound that A. yamamai fibroin gene has expressed in some of the transgenic B. moti. The existence of YAC sequence in the F2 progeny of transgenic B. morisuggested that YAC is a medium for the transfer of fibroin gene. There is only one copy of A. yam. Ami firoin gene in the haploid of F2 progeny. |
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| Keywords: | Bombyx mori Antheraea yamamai fibroin gene yeast artificial chromosome |
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