桔小实蝇线粒体基因组全序列及其分析

桔小实蝇Bactrocera dorsalis线粒体基因组全序列对研究实蝇分子系统进化具有重要意义。本研究通过DNA测序和克隆技术，对桔小实蝇mtDNA全序列进行了测定和分析。结果表明：桔小实蝇线粒体基因组全长15 915 bp（GenBank序列号: DQ845759）。基因组碱基组成为39.3%A，16.2%C，10.2%G，34.3%T，由13个蛋白编码基因、22个tRNA基因、2个rRNA基因以及一个非编码的控制区域（A+T-rich区）组成。7个蛋白编码基因和13个tRNA基因从J链编码，其余6个蛋白编码基因和9个tRNA基因从N链编码。位于J链上的蛋白编码基因具有近似的A、T含量，而位于N链上的蛋白编码基因的A的含量明显高于T的含量。以mtDNA COⅠ基因为例，比较了桔小实蝇与其他14种实蝇的亲缘关系，结果显示其与同亚属（果实蝇亚属Bactrocera）内的其他近缘种相互间的同源性很高。

Sequencing and analysis of the complete mitochondrial genome of the Oriental fruit fly, Bactrocera dorsalis (Hendel) (Diptera: Tephritidae)

The complete mitochondrial genome of the Oriental fruit fly, Bactrocera dorsalis is important for general molecular and evolutionary studies in Tephritidae. Via sequencing and cloning the target DNA sequences, a complete mitochondrial genome sequence of B. dorsalis from the Chinese population was determined and analyzed. The results indicated that the complete mitochondrial genome of B. dorsalis is a circular molecule of 15 915 nucleotides (GenBank accession no.: DQ845759). Its overall composition is 39.3%A, 16.2%C, 10.2%G, and 34.3%T, and the gene content includes 22 tRNAs, 2 rRNAs and a long non-coding region (A+T-rich region). Seven protein genes and 13 tRNA genes are transcribed from J strand, and the other 6 protein genes and 9 tRNA genes are transcribed genes from N strand. Protein coding genes on J strand show a fairly similar A% and T%, whereas those encoded on N strand have a higher proportion of A than T. The similarity analysis of mtDNA COⅠ genes between B. dorsalis and the other 14 fruit flies showed that there was a high similarity among B. dorsalis and the other species belonging to the same subgenus Bactrocera.