四种去垢剂对棉铃虫微粒体细胞色素P450的增溶与变性作用

为分离纯化棉铃虫的细胞色素P450，比较了4种去垢剂对棉铃虫微粒体P450的增溶与变性作用。结果表明：CHAPS (3-(3-cholamidopropyl)- dimethylammonio]-1-propanesulfonate)能有效地增溶中肠和脂肪体微粒体P450，而Lubrol PX（聚氧乙烯十二烷基乙醇醚）、Emulgen 911（聚氧乙烯壬基苯酚醚）和胆酸钠的增溶效果较差；CHAPS对中肠和脂肪体微粒体P450的最适增溶浓度分别为0.5%和0.5%～0.8%; 终浓度为0.5%时, 4种去垢剂对中肠和脂肪体微粒体P450的变性作用不明显。

Effects of four detergents on solubilization and denaturation of microsomal cytochrome P450s in Helicoverpa armigera

To facilitate the separation and purification of cytochrome P450s in the cotton bollworm, Helicoverpa armigera, the effects of 4 detergents on the solubilization and denaturation of microsomal P450s from this pest were examined. The results showed that CHAPS efficiently solubilized the microsomal P450 of midgut and fatbody, while Lubrol PX, Emulgen 911 and sodium cholate were less efficient. The suitable solubilization concentrations of CHAPS for microsomal P450s of midgut and fatbody were 0.5% and 0.5%-0.8% respectively. Denaturation effects of CHAPS, Lubrol PX, Emulgen 911 and sodium cholate at the concentration 0.5% on microsomal cytochrome P450s of midgut and fatbody were insignificant.