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| 粉纹夜蛾颗粒体病毒增效基因3'端2.5 kb片段在大肠杆菌中的表达 | |
| 引用本文： | 袁哲明,孟小林,刘树生.粉纹夜蛾颗粒体病毒增效基因3'端2.5 kb片段在大肠杆菌中的表达[J].昆虫学报,2001,44(2):155-160. |
| 作者姓名： | 袁哲明 孟小林 刘树生 |
| 作者单位： | 1. 湖南农业大学作物学博士后流动站, 2. 武汉大学病毒学研究所, 3. 浙江大学应用昆虫学研究所, |
| 基金项目： | 国家自然科学基金资助项目! (395 70 0 34) |
| 摘 要： | 将粉纹夜蛾Trichoplusia ni颗粒体病毒增效基因3'端2.5 kb片段插入pQE-31中构建了重组表达载体pQE/enhancin，转化大肠杆菌M15(pREP4)在IPTG诱导下成功表达出分子量约为96 kD的融合蛋白并命名为P96。初步纯化的P96显示了明显的增效活性，可提高棉铃虫核型多角体病毒对棉铃虫3龄幼虫感染死亡率27.40%～34.50%，缩短LT50 1.9天以上。 |
| 关 键 词： | 粉纹夜蛾颗粒体病毒 增效基因 克隆 表达 生物测定 |
| 文章编号： | 0454-6296(2001)02-0155-06 |
| 修稿时间： | 1999年5月4日 |
Cloning and expression of 2.5 kb enhancin gene from Trichoplusia ni granulosis virus in Escherichia coli |
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| YUAN Zhe\|ming ,MENG Xiao lin ,LIU Shu sheng.Cloning and expression of 2.5 kb enhancin gene from Trichoplusia ni granulosis virus in Escherichia coli[J].Acta Entomologica Sinica,2001,44(2):155-160. | |
| Authors: | YUAN Zhe\|ming MENG Xiao lin LIU Shu sheng |
| Institution: | YUAN Zhe\|ming 1,MENG Xiao lin 2*,LIU Shu sheng 1 |
| Abstract: | The 2.5 kb fragment of enhancin gene in 3' terminal from Trichoplusia ni granulosis virus was inserted into vector pQE-31 and expressed successfully in Escherichia coli M15 (pREP4). The synergy of the expression product (P96) on Helicoverpa armigera nuclear polyhedrosis virus (HaNPV) against the 3rd instar larvae of H. armigera was also studied. The results indicated that the percentage of accumulated mortality of the larvae increased by 27.40%～34.50% on the 11th day postinfection and the median lethal time decreased at least by 1.9 days in HaNPV+P96 treated compared with those in HaNPV treated. |
| Keywords: | Trichoplusia ni granulosis virus enhancin gene cloning expression bioassay |
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