五种常见嗜尸性蝇类的分子鉴定

为了解决法医学人员对于嗜尸性蝇类的鉴定难题，对中山市、广州市及西安市5种常见嗜尸性蝇类共17个样本，对其线粒体COⅠ基因的348 bp大小片段进行了RFLP和DNA序列分析，采用ABI377测序仪测序，DNASTAR软件预测限制性位点，DdeⅠ, DraⅠ和HinfⅠ3种限制性内切酶消化，非变性聚丙烯酰胺凝胶电泳检测酶切结果，MEGA3.0软件包进行序列分析和构建系统发育树。结果表明： 采用mtDNA扩增结合银染技术和DNA序列分析，均可以方便快捷地进行上述三地5种常见嗜尸性蝇类的种类鉴定。本文结果为法医昆虫学中嗜尸性昆虫DNA鉴定数据库的建立提供了数据资料。

Molecular identification of five common species of necrophagous flies in China

In order to solve the difficulty of the identification of necrophagous flies faced by forensic pathologists and technicians, the feasibility of species identification based on a 348 bp region of the mitochondrial COⅠ (cytochrome oxidase subunit Ⅰ)  gene using RFLP and analysis of DNA was evaluated. Samples were collected in Zhongshan, Guangzhou and Xi'an. The 348 bp region of the COⅠ gene was amplified and sequenced using forward and reverse primers. Cleavage sites for several restriction endonucleases were searched using the DNASTAR. PCR products were digested with restriction endonuclease DdeⅠ, DraⅠ and HinfⅠ, and then separated on native polyacrylamide gels. Evolutionary tree and evolutionary divergence analysis were performed using MEGA 3.0. The analysis indicated that the methods could be used to identify common necrophagous flies. The results provided basis for further research, especially for the setup of mtDNA database of insects of forensic importance.