家蝇中乙酰胆碱酯酶、羧酸酯酶和多功能氧化酶活性与抗药性的关系

本文对几种抗药性和敏感性家蝇品系的乙酰胆碱酯酶(AChE)、羧酸酯酶及多功能氧化酶(MFO)进行了测定.结果表明:①抗药性品系和敏感性品系的AChE活力差异不大, 有机磷抗性品系的AChE对对氧磷的不敏感性比敏感性家蝇明显增大.②某些抗药性家蝇的羧酸酯酶活力比敏感性家蝇大.③抗药性家蝇的MFO活力(O-脱甲基和环氧化)比敏感性家蝇均有不同程度的增高.④二氯苯醚菊酯抗性家蝇对有机磷有负交互抗性.

THE ACTIVITIES FOR ACETYLCHOLINESTERASE, CARBOXYLESTERASE AND MIXED-FUNCTION OXIDASE IN RELATION TO PESTICIDE RESISTANCE IN THE HOUSEFLY

The activities of acetylcholinesterase (AChE), carboxylesterase and mixed ftmction oxidase (MFO) were determined in different strains of the Lousefly Musea domestica vidn?bred in the laboratory. The results indicated that the resistance to organophosphorus pesticides is caused by the insensitivity of the AChE to these pesticides and higher activity of the MFO. The I50 of AChE of the resistant flies for paraoxon is 2.4-4.2 times higher than that of the susceptible ones. The ratios of O dcmcthyl and epoxide activities of crude MFO extracts of the rpsistant flies to that of the susceptible ones have been found to be 2.7-4.2 and 2.3-3.5 respectively. Higher activities of aliesterase, carboxylesterase and MFO are observed in the flies resistant to DDT and permethrin respectively.