转新型双价抗虫基因杨树对光肩星天牛及美国白蛾的抗性鉴定

为明确双价基因chitinase-BmkIT对鞘翅目害虫光肩星天牛及鳞翅目害虫美国白蛾的抗虫效果, 分别以转该双价基因的杨树茎干和叶片为材料，采用室内茎干接虫和叶片接虫方法鉴定其对光肩星天牛Anoplophora glabripennis和美国白蛾Hyphantria cunea幼虫的抗性。结果表明：转基因株系132-2，144-1，123-1对美国白蛾幼虫的致死作用和生长发育影响显著高于取食未转化叶片的处理（P＜0.05），且对其后代产生了影响，表现为幼虫不能化蛹或化蛹后不能羽化或羽化不正常；但供试的8个转基因株系对光肩星天牛幼虫无明显的致死和生长抑制作用，表现为幼虫死亡率和体重均与取食非转化茎干幼虫无显著差异（P＞0.05）。结果提示，双价基因chitinase-BmkIT可作为杨树鳞翅目害虫的抗虫基因源，并且对延缓害虫抗性的产生具有一定的意义。

Bioassays of resistance of transgenic poplar with novelbinary insect-resistant genes to Anoplophora glabripennis (Coleoptera: Cerambycidae) and Hyphantria cunea (Lepidoptera: Arctiidae)

To verify the resistance of binary genes chitinase-BmkIT to larvae of Coleopterous insect Anoplophora glabripennis and Lepidopterous insect Hyphantria cunea,in vitro insect-resistant bioassays were conducted,where the 1st-instar larvae of A.glabripennis were inoculated into poplar stems and those of H.cunea onto fresh poplar leaves.The results showed that transgenic clone 132-2,144-1 and 123-1 had significant lethal and inhibitory effects on H.cuneal larvae compared with the untransformed control(P<0.05).Most H.cunea larvae were not able to develop into pupae,some pupae were not able to emerge,and some emerged moths were abnormal with imperfect wings which died soon without mating.However,the differences in larval mortality and body weight of A.glabripennis were not significant compared with those of the untransformed control,indicating that transgenic stems had little influence on the survival and development of A.glabripennis larvae.The results suggested that the insect-resistant gene combination could not only be used as one of the complementary alien gene sources for resisting Lepidopterous pests but also contributed to reducing the risk of development of insect resistance produced by using single insect toxin gene.