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| 棉铃虫化学感受蛋白HarmCSP6二聚体的组织表达分析及气味结合特征 | |
| 引用本文： | 张天涛,邹朗云,李科明,冯纪年,张永军,郭予元.棉铃虫化学感受蛋白HarmCSP6二聚体的组织表达分析及气味结合特征[J].昆虫学报,2011,54(6):615-622. |
| 作者姓名： | 张天涛 邹朗云 李科明 冯纪年 张永军 郭予元 |
| 作者单位： | 1. 西北农林科技大学植物保护科技学院,陕西杨陵712100;中国农业科学研究院植物保护研究所植物病虫害生物学国家重点实验室,北京100193 2. 中国农业科学研究院植物保护研究所植物病虫害生物学国家重点实验室,北京,100193 3. 西北农林科技大学植物保护科技学院,陕西杨陵,712100 |
| 基金项目： | 国家自然科学基金项目(30871640); 国家科技支撑计划项目(2006BAD08A07) |
| 摘 要： | 为了研究棉铃虫Helicoverpa armigera(Hübner)化学感受蛋白(chemosensory proteins,CSPs)在嗅觉识别过程中的功能,本研究克隆并在原核细胞中表达了棉铃虫化学感受蛋白HarmCS6,Western blot和快速液相色谱(fast protein liquid chromat... |
| 关 键 词： | 棉铃虫 化学感受蛋白 原核表达 荧光定量PCR 二聚体 结合实验 |
| 收稿时间： | 2011-01-14; |
Expression profiling and binding characterization of dimeric chemosensory protein 6 (HarmCSP6) in Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) |
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| ZHANG Tian-Tao,ZOU Lang-Yun,LI Ke-Ming,FENG Ji-Nian,ZHANG Yong-Jun,GUO Yu-Yuan.Expression profiling and binding characterization of dimeric chemosensory protein 6 (HarmCSP6) in Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae)[J].Acta Entomologica Sinica,2011,54(6):615-622. | |
| Authors: | ZHANG Tian-Tao ZOU Lang-Yun LI Ke-Ming FENG Ji-Nian ZHANG Yong-Jun GUO Yu-Yuan |
| Institution: | ZHANG Tian-Tao1,2,ZOU Lang-Yun2,LI Ke-Ming1,FENG Ji-Nian1,ZHANG Yong-Jun2,*,GUO Yu-Yuan1,* (1.Northwest Agriculture and Forestry University,Yangling,Shaanxi 712100,China,2.State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China) |
| Abstract: | In order to study the functions of chemosensory proteins (CSPs) of Helicoverpa armigera (Hübner) in olfactory recognition, we cloned and expressed the chemosensory protein HarmCSP6 of H. armigera in prokaryotic cell. This protein was expressed in the form of dipolymer, which was certified by Western blotting and fast protein liquid chromatography (FPLC). The results of expression profiles showed that the HarmCSP6 gene was highly expressed in male and female antennae, and was expressed to a certain extent in female legs and wings. The binding experiment with 22 kinds of odorant chemicals indicated that HarmCSP6 could bind obviously with aldehydes and terpenes. By studying the binding affinity, some special binding odorants can be selected. This may provide the theoretical basis for developing attractants and repellents of the cotton bollworm. |
| Keywords: | Helicoverpa armigera chemosensory protein prokaryotic expression fluorescence quantitative PCR dipolymer binding experiment |
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