松毛虫赤眼蜂两性生殖品系和孤雌产雌品系在不同品种柞蚕卵中的寄生和生长发育表现

【目的】明确两性生殖品系和孤雌产雌品系松毛虫赤眼蜂Trichogramma dendrolimi在不同品种柞蚕Antheraea pernyi卵中的寄生及发育表现，为以柞蚕卵为替代寄主更好地规模化繁育赤眼蜂提供依据。【方法】测定两性生殖品系和孤雌产雌品系雌蜂在6个品种柞蚕卵［抗大(KD)、大四(DS)、高新(GX)、988(NEE)、青大(QD)和特大(TD)］上的寄生率、子代蜂窝蜂数(单窝羽化子代蜂数)和子代蜂雌性比等生物学指标，以及6个品种柞蚕卵的质量指标(单卵湿重、蛋白质含量、总糖含量和甘油三酯含量)；利用主成分分析揭示柞蚕卵质量指标与赤眼蜂生物学指标间的相关性。【结果】NEE品种柞蚕卵育出的两性品系松毛虫赤眼蜂子代蜂个体显著大于除KD和QD品种以外的其他柞蚕品种卵育出的两性品系子代蜂。柞蚕品种对两性品系松毛虫赤眼蜂的寄生率、子代蜂窝蜂数和子代蜂雌性比均无显著影响。在孤雌产雌品系松毛虫赤眼蜂中，KD品种柞蚕卵育出的松毛虫赤眼蜂子代蜂窝蜂数最多，显著高于NEE和QD品种育出的子代蜂窝蜂数，但与DS, GX和TD品种育出的子代蜂窝蜂数相比无显著差异。柞蚕品种对孤雌产雌品系松毛虫赤眼蜂的寄生率和子代蜂个体大小均无显著影响。TD品种柞蚕卵的蛋白质含量最高，显著高于除GX品种以外的柞蚕品种。KD品种柞蚕卵的总糖含量和QD品种柞蚕卵的甘油三酯含量最高，均分别显著高于其余柞蚕品种。主成分分析表明，两性品系松毛虫赤眼蜂寄生率、柞蚕卵蛋白质含量和甘油三酯含量均与柞蚕卵总糖含量呈负相关；两性品系松毛虫赤眼蜂子代蜂个体大小和柞蚕卵甘油三酯含量与单卵湿重呈负相关；孤雌产雌品系松毛虫赤眼蜂寄生率和柞蚕卵甘油三酯含量与松毛虫赤眼蜂子代蜂窝蜂数、柞蚕卵总糖含量和单卵湿重呈负相关；孤雌产雌品系松毛虫赤眼蜂子代蜂个体大小和柞蚕卵总糖含量与蛋白质含量呈负相关。【结论】本研究通过筛选适宜两性生殖品系和孤雌产雌品系赤眼蜂繁育的柞蚕卵品种，初步明确了卵内主要营养指标与子代蜂生物学指标间的相关性。研究结果为利用柞蚕卵更好地规模化繁育松毛虫赤眼蜂提供依据与数据支撑。

Parasitism and development performance of bisexual and thelytokous lines ofTrichogrammadendrolimi(Hymenoptera: Trichogrammatidae) on eggs of different strains ofAntheraeapernyi (Lepidoptera: Saturniidae)

【Aim】 To determine the parasitism and development performance of bisexual andthelytokous lines of Trichogramma dendrolimi on eggs of different strains of Antheraeapernyi, so as to provide the basis for the mass-rearing of richogrammatid parasitoidsusing A. pernyi eggs as the alternative host. 【Methods】 The biological parametersincluding parasitism rate, brood size of offspring (number of offspring wasps emerged froma single brood), and proportion of female offspring of bisexual and thelytokous lines of T.dendrolimi on eggs of six strains of A. pernyi ［Kangda (KD), Dasi (DS), Gaoxin (GX), 988(NEE), Qingda (QD), and Teda (TD)］ were detected, and the quality parameters of eggs ofthe six strains of A. pernyi (wet weight per egg, protein content, total carbohydratecontent and triglyceride content) were determined. The correlation between the qualityparameters of A. pernyi eggs and the biological parameters of T. dendrolimi was detected byprincipal component analysis. 【Results】 The body size of offspring of the bisexual waspsof T. dendrolimi developed on eggs of the NEE strain of A. pernyi were significantly higherthan those developed on eggs of the other strains of A. pernyi except for the KD and QDstrains. The parasitism rate, brood size of offspring, and proportion of female offspringof the bisexual wasps of T. dendrolimi were not significantly influenced by the strain ofA. pernyi. The brood size of offspring of thelytokous wasps of T. dendrolimi developed oneggs of the KD strain of A. pernyi was the highest, being significantly higher than thosedeveloped on eggs of the NEE and QD strains of A. pernyi, but without significantdifference with those developed on eggs of the DS, GX, and TD strains of A. pernyi. Theparasitism rate and brood size of offspring of the thelytokous wasps of T. dendrolimi werenot significantly influenced by the strain of A. pernyi. The protein content in eggs of theTD strain of A. pernyi was the highest, significantly higher than those in eggs of theother strains of A. pernyi except for the GX strain. The total carbohydrate content in eggsof the KD strain of A. pernyi and the triglyceride content in eggs of the QD strain of A.pernyi were the highest, significantly higher than those in eggs of the other strains,respectively. The principal component analysis showed that the parasitism rate of bisexualwasps of T. dendrolimi, and the protein content and triglyceride content in A. pernyi eggswere negatively correlated with the total carbohydrate content in A. pernyi eggs. The bodysize of offspring of the bisexual wasps of T. dendrolimi was negatively correlated with thewet weight per egg of A. pernyi. The parasitism rate of the thelytokous wasps of T.dendrolimi and the triglyceride content in A. pernyi eggs were negatively correlated withthe brood size of offspring of T. dendrolimi, the total carbohydrate content, and the wetweight per egg of A. pernyi. The body size of offspring of thelytokous wasps of T.dendrolimi and the total carbohydrate content in A. pernyi eggs were negatively correlatedwith the protein content in A. pernyi eggs. 【Conclusion】 This study determined thesuitable strain of A. pernyi eggs for the mass-rearing procedure of bisexual andthelytokous lines of T. dendrolimi and revealed the relationship between the nutritiveindexes of A. pernyi eggs and the biological parameters of T. dendrolimi. The resultsprovide basic data for improving mass-rearing of T. dendrolimi.