芫菁斑蝥素对喉癌细胞和胃癌细胞的抑制作用

【目的】 研究提取自眼斑芫菁Mylabris cichorii (Linnaeus)体内的斑蝥素对人喉癌HEP-2细胞和人胃癌BGC-823细胞的抑制、以及对细胞周期分布的影响。【方法】 将斑蝥素作用于经体外培养的人喉癌HEP-2细胞和人胃癌BGC-823细胞, 采用MTT法进行体外细胞抑制实验, 测定斑蝥素对这2种癌细胞生长的抑制率与剂量效应；采用流式细胞术测定斑蝥素处理的人喉癌HEP-2细胞的细胞周期；并通过光学显微镜观察其细胞形态学改变。【结果】 斑蝥素浓度为1.28 μmol/L时, 对HEP-2细胞有显著抑制作用, 且随药物浓度升高其抑制作用增强, 呈剂量效应关系, 抑制中浓度为2.88 μmol/L；斑蝥素浓度为20.4 μmol/L时, 对BGC-823细胞有显著抑制作用, 且随药物浓度升高其抑制作用增强, 呈剂量效应关系, 抑制中浓度为54.85 μmol/L。用浓度1.44和2.88 μmol/L的斑蝥素处理HEP-2细胞24 h后, G2-M期分布从8.21%增加到22.29%, S期细胞分布从14.33%增加到21.61%, 且随药物浓度升高其阻滞作用增加, 呈剂量效应关系。G0-G1期细胞分布都有所降低, 从77.45%降低到56.10%, G0-G1期峰前无显著的亚二倍体峰出现, 说明斑蝥素未能够诱导HEP-2细胞发生凋亡。光镜检查显示：HEP-2细胞可出现细胞收缩、胞膜突出、核碎裂等现象。【结论】 斑蝥素对治疗喉癌的效果可能较为理想, 而对胃癌的作用则不明显。

Inhibitory effect of cantharidin from meloids on laryngeal carcinoma cell lines and gastric carcinoma cell lines

Objective] To study the inhibitory effect of cantharadin extracted from meloids Mylabris cichorii on human laryngeal carcinoma HEP-2 cell lines and human gastric carcinoma BGC-823 cell lines, and the influence on cell cycle. Methods ] Human laryngeal carcinoma HEP-2 cell lines and human gastric carcinoma BGC-823 cell lines were cultured by cell culture technique. MTT colorimetry was used to evaluate the inhibitory effect of cantharidin on cell grouth. Flow cytometry was used to measure the induction of cell cycle arrest. Microscope was used to observe the morphological changes of the cells. Results ] Cantharidin displayed the inhibitory effect on the growth of HEP-2 cells at the concentration of 1.28 μmol/L. The inhibition was in a dose-dependent manner with the IC_(50) value of 2. 88 μmol/L. And cantharidin displayed the inhibitory effect on the growth of BGC-823 cells at the concentration of 20.4 μmol/L. The inhibition was in a dose-dependent manner with the IC_(50) value of 54. 85 μmol/L. After HEP-2 cells were treated with 1.44 and 2. 88 μmol/L cantharidin for 24 h, their distributions in G_2-M phase and S phase increased from 8.21% to 22.29% and from 14.33% to 21.61% , respectively, while that in G_0-G_1 phase decreased from 77.45% to 56. 10% and no apoptosis was induced. Cell shrinkage, membrane budding, and chromosome lagging of some HEP-2 cells were observed under microscope. Conclusions ] The results suggest that cantharidin may have higher application significance to the therapy of laryngeal cancer, as compared with that of gastric cancer. Cantharidin can inhibit the growth of human laryngeal carcinoma HEP-2 cell lines.