转Bt-cry1Ab玉米花粉对异色瓢虫生长发育及体内三种代谢酶活性的影响

用转Bt-cry1Ab基因玉米花粉饲喂异色瓢虫，初步研究了转基因玉米花粉对瓢虫的影响。结果显示，异色瓢虫取食混有适量蚜虫的转Bt-cry1Ab基因玉米花粉时与取食混有适量蚜虫的非转基因亲本玉米花粉时相比，各虫态发育历期没有显著差异；取食转Bt-cry1Ab基因玉米花粉对异色瓢虫的体重增加无明显影响。多数龄期内取食转基因玉米花粉的异色瓢虫体内的α-乙酸萘酯酶活性、乙酰胆碱酯酶活性以及谷光甘肽-S-转移酶活性与对照组相比没有显著差异。用酶联免疫（ELISA）方法在取食转Bt-cry1Ab基因玉米花粉的瓢虫体内未检测到Bt杀虫蛋白。转Bt-cry1Ab玉米花粉对异色瓢虫生长发育没有显著负面影响，初步证明Bt玉米MON810花粉对异色瓢虫是安全的。

Effects of transgenic Bt-cry1Ab corn pollen on the growth and development and the activity of three metabolic enzymes in Harmonia axyridis (Pallas) (Coleoptera: Coccinellidae)

In order to explore the effects of transgenic Bt-cry1Ab corn pollen on the multicolored Asian lady beetle (MALB), Harmonia axyridis (Pallas), a series of experiments were conducted with a non-Bt isoline as the control in the laboratory. The H. axyridis beetles were fed with transgenic Bt-cry1Ab corn pollen mixed with a certain proportion of pea aphids Acyrthosiphon pisum (Harris) in the laboratory, and changes in their developmental duration and body weight at different stages were observed. The activity changes of three metabolic enzymes (α-naphthylacetate esterase, acetylcholinesterase and glutathione-S-transferase) in H. axyridis reared on transgenic Bt-cry1Ab corn pollen were also determined. Moreover, we monitored the sediment of Bt-Cry1Ab toxin protein in the bodies of H. axyridis by ELISA method. The results indicated that there was no marked difference in the developmental duration at each stage between these beetles fed on  transgenic Bt-cry1Ab corn pollen and those on non_Bt isoline corn pollen. The transgenic Bt-cry1Ab corn pollen also had no notable side effects on the body weight of H. axyridis  at different stages. The activities of metabolic enzyme activities in H. axyridis fed on transgenic Bt-cry1Ab corn pollen changed slightly compared with the control, which had no visible effect on the growth of H. axyridis. The sediment of Bt-Cry1Ab toxin protein in the bodies of H. axyridis at different developmental stages was so scarce that it could not been detected by ELISA method. It was concluded that there was no significantly harmful effect of transgenic Bt-cry1Ab corn on the growth and development of the beetle.