湘西黑藤藤中对小菜蛾幼虫有拒食活性化合物的分离与鉴定

为探明湘西黑藤Derric cavalerei 藤中对小菜蛾Plutella幼虫有拒食活性的化合物及其拒食活性,通过活性跟踪、利用柱色谱、借助核磁共振和质谱分离,鉴定了其拒食成分,并使用叶碟法测定各化合物的拒食活性.结果包括:首次从该植物藤中得到4个化合物,即isolonchcarpin,magnificol,oval...

Isolation and identification of antifeeding compounds from vines of Derris cavaleriei(Leguminosae)against Plutella xylostella(Lepidoptera:Yponomeutidae)larvae

In order to determine the active compounds from Derris cavaleriei vines and their antifeedant activities against Plutella xylostella larvae,the active compounds were isolated by activity-guided fractionation with column chromatography and identified on the basis of MS and NMR data,and the antifeedant activity was determined by sandwich method.The results included that four compounds i.e.isolonchcarpin,magnificol,ovaliflavanone and millettocalyxin C had been isolated from the plant vines for the first time,and isolonchcarpin,magnificol and millettocalyxin C exhibited antifeeding activity,with the AFC50 values of 25.5,92.8 and 1 15.9 mg/L against the 3rd instar larvae of P.xylostella at 48 h after treatment,respectively,suggesting that the three compounds are antifeedant compounds against P.xylostella derived from the plant vines.The elucidation of these chemicals is important not only for understanding the insect-plant relationships.but also for their potential in P.xylostella control.