海南地区螺旋粉虱种群mtDNA-COI和rDNA-ITS1基因的序列及系统发育分析

螺旋粉虱Aleurodicus dispersus Russell在海南是一种重要的农林害虫。本研究对海南地区不同螺旋粉虱种群的mtDNA-COI和rDNA-ITS1基因片段进行了测定并对其系统发育进行了分析。mtDNA-COI和rDNA-ITS1的序列比较发现， 海南16个县市螺旋粉虱种群的mtDNA-COI序列基本一致， 只有文昌番石榴Psidium guajava种群的序列发生变异， 在476 bp位点的碱基C突变为碱基T。不同螺旋粉虱种群的rDNA-ITS1序列也无差异。基于mtDNA COI序列的不同螺旋粉虱种群的系统发育树表明， 已传入我国海南地区的螺旋粉虱未发生明显的遗传分化； 研究还发现海南的螺旋粉虱种群与台湾的种群遗传距离最近， 表明海南地区的螺旋粉虱可能由台湾传入的。基于mtDNA-COI和rDNA-ITS1序列构建的不同粉虱种群的系统发育树表明， 粉虱科分为复孔粉虱亚科（Aleurodicinae）和粉虱亚科（Aleyrodinae）， 这一结果与其他研究结果一致。

Sequence and phylogenetic analyses of mtDNA-COI and rDNA-ITS1 genes in populations of Aleurodicus dispersus (Hemiptera: Aleyrodidae) in Hainan,southern China

The spiraling whitefly (SWF), Aleurodicus dispersus Russell, is an important agricultural and forest pest in Hainan province, southern China. In this study, we sequenced and analyzed the gene sequences of mtDNA-COI and rDNA-ITS1 genes within A. dispersus populations from 16 districts in Hainan. The results showed that mtDNA-COI gene sequences of the spiraling whitefly populations from different regions of Hainan were identical, except Wenchang sample coming from Psidium guajava carrying one variable position (C-T) at position 476 bp in mtDNA-COI gene. Analyses of the rDNA-ITS1 gene showed that the ITS1 sequences of all the A. dispersus populations were consistent. The phylogeny trees of A. dispersus populations based on mtDNA-COI gene showed that fairly low genetic variation existed among the different populations sampled in various locations. Taiwan population and Hainan population had a fairly closed relationship according to the phylogenetic trees, suggesting that the invaded A. dispersus in Hainan likely originated from Taiwan. Aleyrodidae could be divided into two subfamilies, i. e., Aleurodicinae and Aleyrodinae, according to the phylogenetic trees of different whitefly species based on mtDNA-COI and rDNA-ITS1 gene sequences, which is consistent with the results of former studies.