Wolbachia在我国广赤眼蜂种群内的感染

Wolbachia是广泛分布于节肢动物生殖组织内的一类细胞内共生菌，它属于原细菌的α亚类，能够通过调控寄主的生殖活动而促进其在寄主种群中的扩散。通过对wsp 基因的克隆及PCR-RFLP分析确定了Wolbachia在我国广赤眼蜂种群内的存在，并发现有2种Wolbachia 菌系的感染，命名为wEvaA和wEvaB。经过克隆分离得到了这2种Wolbachia的wsp基因序列，在GenBank的登录号为AY390279和 AY390280 ，并由基于wsp基因的聚类树中发现，这两种Wolbachia菌系均属于A组。

Infection of the endosymbiont Wolbachia in population of Trichogramma evanescens in China

Wolbachia, belonging to α-Proteobacteria, is a commonly widespread group of intracellular symbiotic bacteria found in reproductive tissues of arthropods. The bacteria may spread in host populations through regulation of host reproduction. In this study, the presenc e of Wolbachia was detected in a natural population of Trichogramma evanescens in China based on the wsp gene cloning and PCR-RFLP analysis. Two wsp genes of Wolbachia infecting T. evanescens were amplified and se quenced, with their GenBank accession numbers as AY390279 and AY390280 Also two new Wolbachia strains were identified and named as wEvaA and wEvaB. Through constructing and analysing the phylogenic tree of Wolbachia strains in Trichogramma spp. Based on wsp gene, we found these two strains belonged to A group.