| 黄粉虫抗茵肽TmAMP3在大肠杆菌中的高效表达及活性检测 |
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| 引用本文： | 唐馨,毛新芳,热西力·克来木,刘忠渊.黄粉虫抗茵肽TmAMP3在大肠杆菌中的高效表达及活性检测[J].昆虫学报,2011,54(10):1111-1117. |
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| 作者姓名： | 唐馨 毛新芳 热西力·克来木 刘忠渊 |
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| 作者单位： | 新疆大学生命科学与技术学院,新疆生物资源基因工程重点实验室,乌鲁木齐830046 |
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| 基金项目： | 新疆维吾尔自治区科技支疆项目(200991130); 自治区自然科学基金项目(200821120); ]博士启动基金项目(BS090126) |
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| 摘 要： | 昆虫抗菌肽具有广谱抗菌活性,克隆黄粉虫Tenebrio molitor抗菌肽基因,进行原核表达和活性检测,为昆虫抗菌肽推广应用奠定一定基础.根据GenBank公布的黄粉虫抗菌肽序列设计特异引物,以RT-PCR法从黄粉虫体内克隆了抗菌肽基因TmAMP3,将其亚克隆至pET-30a表达载体中,转化到大肠杆菌Escheric...
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| 关 键 词： | 黄粉虫 抗菌肽 高效表达 蛋白纯化 抗菌活性 |
High-level expression and function assay of antimicrobiol peptide TmAMP3 of Tenebrio molitor (Tenebrionidae, Coleoptera) in Escherichia coli |
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| TANG Xin,MAO Xin-Fang,Kelaimu REXILI,LIU Zhong-Yuan.High-level expression and function assay of antimicrobiol peptide TmAMP3 of Tenebrio molitor (Tenebrionidae, Coleoptera) in Escherichia coli[J].Acta Entomologica Sinica,2011,54(10):1111-1117. |
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| Authors: | TANG Xin MAO Xin-Fang Kelaimu REXILI LIU Zhong-Yuan |
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| Institution: | TANG Xin,MAO Xin-Fang,Kelaimu REXILI,LIU Zhong-Yuan (Xinjiang Key Laboratory of Biological Resources and Genetic Engineering,College of Life Science and Technology,Xinjiang University,Urumqi 830046,China) |
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| Abstract: | Insect antimicrobial peptides (AMPs) have wide antimicrobial spectrum. Cloning and prokaryotic expression of antimicrobial peptide TmAMP3 gene from Tenebrio molitor could provide a basis for further application of this antimicrobial peptide. TmAMP3 was amplified by RT-PCR using a pair of specific primers designed according to the relevant nucleotide sequence from GenBank. The gene TmAMP3 was subcloned directionally into the prokaryotic expression vector pET-30a and transformed into Escherichia coli BL21. Af... |
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| Keywords: | Tenebrio molitor antimicrobial peptide high-level expression protein purification antibacterial activity |
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