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| 苹果蠹蛾热激蛋白Hsp90基因的克隆及热胁迫下的表达分析 | |
| 引用本文： | 申建茹,李明福,陈乃中,王进军,万方浩.苹果蠹蛾热激蛋白Hsp90基因的克隆及热胁迫下的表达分析[J].昆虫学报,2011,54(11):1236-1248. |
| 作者姓名： | 申建茹 李明福 陈乃中 王进军 万方浩 |
| 作者单位： | 1. 西南大学植物保护学院,重庆400715;中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193 2. 中国检验检疫科学研究院动植物检疫研究所,北京,100029 3. 西南大学植物保护学院,重庆,400715 |
| 基金项目： | 国家“十一五”科技支撑计划项目(2006BAD08A17); 公益性行业(农业)科研专项经费项目(200903042) |
| 摘 要： | 世界检疫性害虫苹果蠹蛾Cydia pomonella是一种温度耐受可塑性很高的物种.本研究针对温度波动可能导致其耐热性增强的科学问题,采用生测法鉴定了苹果蠹蛾实验种群的高温耐受阈值,采用同源克隆、RACE和实时荧光定量PCR (RT-qPCR)等方法研究了苹果蠹蛾热激蛋白Hsp90基因的应激表达对耐热性的重要作用.高温... |
| 关 键 词： | 苹果蠹蛾 耐热性 热激蛋白 系统发育 热激诱导 相对表达模式 |
Cloning and analysis of the expression characteristics under heat stress of Hsp90 gene in the codling moth,Cydia pomonella(Lepidoptera:Tortricidae) |
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| SHEN Jian-Ru , LI Ming-Fu , CHEN Nai-Zhong , WANG Jin-Jun , WAN Fang-Hao.Cloning and analysis of the expression characteristics under heat stress of Hsp90 gene in the codling moth,Cydia pomonella(Lepidoptera:Tortricidae)[J].Acta Entomologica Sinica,2011,54(11):1236-1248. | |
| Authors: | SHEN Jian-Ru LI Ming-Fu CHEN Nai-Zhong WANG Jin-Jun WAN Fang-Hao |
| Institution: | SHEN Jian-Ru~1,2,LI Ming-Fu~3,CHEN Nai-Zhong~3,WANG Jin-Jun~1,WAN Fang-Hao~1,*(1.College of Plant Protection,Southwest University,Chongqing 400715,China,2.State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,3.Institute of Animal and Plant Quarantine,Chinese Academy of Inspection and Quarantine,Beijing 100029,China) |
| Abstract: | The codling moth,Cydia pomonella(Lepidoptera:Tortricidae),is a worldwide quarantine pest and possesses the plasticity in thermotolerance ability.The temperature fluctuations may enhance its tolerance to heat stress.In the present study,we determined the critical threshold of tolerance to high temperature by bioassay method in C.pomonella laboratory population,and applied the techniques of homology-based cloning,RACE and real-time quantitative PCR(RT-qPCR) to elucidate the function of Hsp90 responding to hea... |
| Keywords: | Cydia pomonella heat resistance heat shock protein(Hsp) phylogeny heat induction relative expression pattern |
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