| 应用巢式PCR-DGGE技术分析稻虱缨小蜂体内Wolbachia的多样性 |
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| 引用本文： | 刘淑平,王新,徐红星,汤江武,郑许松,杨亚军,吕仲贤.应用巢式PCR-DGGE技术分析稻虱缨小蜂体内Wolbachia的多样性[J].昆虫学报,2011,54(12):1354-1360. |
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| 作者姓名： | 刘淑平 王新 徐红星 汤江武 郑许松 杨亚军 吕仲贤 |
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| 作者单位： | 浙江省农业科学院植物保护与微生物研究所， 杭州 310021 |
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| 基金项目： | 农业部公益性行业科研专项(200903051;201003031); 浙江省自然科学基金重点项目(Z3080437) |
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| 摘 要： | 以采集自中国杭州和菲律宾的稻虱缨小蜂Anagrus nilaparvatae为研究对象,采用巢式PCR扩增Wolbachia的16S rDNA和wsp基因片段,并用DGGE分析稻虱缨小蜂体内Wolbachia的多样性.基于16S rDNA基因的分析结果准确地检测到稻虱缨小蜂体内细菌主要是Acinetobacter sp...
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| 关 键 词： | 稻虱缨小蜂 Wolbachia 多样性 16S rDNA基因 wsp基因 PCR-DGGE |
Diversity of Wolbachia in Anagrus nilaparvatae(Hymenoptera:Mymaridae) analyzed using nested PCR-DGGE |
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| LIU Shu-Ping
,
WANG Xin
,
XU Hong-Xing
,
TANG Jiang-Wu
,
ZHENG Xu-Song
,
YANG Ya-Jun
,
L Zhong-Xian.Diversity of Wolbachia in Anagrus nilaparvatae(Hymenoptera:Mymaridae) analyzed using nested PCR-DGGE[J].Acta Entomologica Sinica,2011,54(12):1354-1360. |
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| Authors: | LIU Shu-Ping WANG Xin XU Hong-Xing TANG Jiang-Wu ZHENG Xu-Song YANG Ya-Jun L Zhong-Xian |
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| Institution: | Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China |
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| Abstract: | The objective of this research is to analyze the diversity of Wolbachia in insects by using nested PCR-DGGE.Samples of Anagrus nilaparvatae,one of dominant egg parasitoids of rice planthoppers in Asian rice growth area,were collected from Hangzhou,China and the Philippines.After the total DNA was extracted,the 16S rDNA and wsp gene fragments of Wolbachia were amplified with nested PCR,and then analyzed using DGGE.The results showed that Wolbachia in A.nilaparvatae were sensitively and exactly detected based... |
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| Keywords: | Anagrus nilaparvatae Wolbachia diversity 16S rDNA gene wsp gene PCR-DGGE |
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