优雅蝈螽雄性附腺的超微结构及其腺管提取物对精子束的作用

为阐明优雅蝈螽Gampsocleis gratiosa Brunner von Wattenwyl雄性附腺的结构与功能的关系， 本文利用透射电镜(transmission electron microscope, TEM)技术研究了优雅蝈螽雄性附腺的超微结构， 利用微分干涉相差显微镜(differential interference contrast microscope, DIC)技术并结合雄性附腺匀浆提取物与精子束在体外的短暂培养， 研究了优雅蝈螽雄性附腺对精子束的作用。结果表明： 优雅蝈螽雄性附腺3类腺管组织结构相似， 腺管管壁为单层上皮细胞， 缺少内表皮， 说明其来源于中胚层。上皮细胞富含粗面内质网、 线粒体、 高尔基体， 具有分泌细胞的特点。腺管管腔中分泌物有4种形态， 即电子透明的物质、 电子致密的颗粒物质、 细纤维状物质以及绒球状物质。上皮细胞的分泌方式主要有2种， 即顶质分泌和局部分泌。乳白短腺管的匀浆提取物参与了帽状精子束解聚的过程， 乳白长腺管和透明腺管的匀浆提取物有维持精子束活性的作用。本研究结果为进一步阐明螽斯雄性附腺的生理功能奠定了基础。

Ultrastructure of male accessory glands and the function of the extract from gland tubules to spermatodesms in Gampsocleis gratiosa (Orthoptera: Tettigoniidae)

To clarify the relation of the structure of male accessory glands from Gampsocleis gratiosa Brunner von Wattenwyl with their functions, we examined the ultrastructure of male accessory glands using transmission electron microscope, and explored their roles in spermatodesms using differential interference contrast microscope. The results revealed that all gland tubules of the male accessory glands are histologically similar. The epithelium is always monolayered and devoid of cuticular intima, suggesting its ...