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长镰管蚜气味结合蛋白OBP3的cDNA克隆和序列分析及其成虫嗅觉行为反应测定
引用本文:范佳,刘勇,陈巨莲,程登发.长镰管蚜气味结合蛋白OBP3的cDNA克隆和序列分析及其成虫嗅觉行为反应测定[J].昆虫学报,2011,54(9):975-981.
作者姓名:范佳  刘勇  陈巨莲  程登发
作者单位:1. 中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193
2. Functional and Evolutionary Entomology,Gembloux Agro-Bio Tech, University of Liege, Passage des Déportés 2, B-5030 Gembloux, Belgium
3. 山东农业大学植物保护学院,山东泰安,271018
基金项目:国家自然科学基金项目(30971920); 国家重大科技专项(2009ZX08012-007B); 国家小麦产业建设体系专项经费(nycytx-03); 中比合作项目(2010DFA32810,PICSHANDONG)
摘    要:枫长镰管蚜Drepanosiphum platanoidis是少数几种不分泌E-β-法尼烯(E-β-farnesene,EBF)的蚜虫之一.ApisOBP3是首个被鉴定的特异结合EBF的昆虫气味结合蛋白,来自于豌豆蚜Acyrthosiphum pisum.本研究通过基因克隆技术,在枫长镰管蚜体内获得2个表达ApisOB...

关 键 词:枫长镰管蚜  气味结合蛋白  报警信息素  E-β-法尼烯(EBF)  cDNA克隆  四臂嗅觉仪
收稿时间:2011-05-03;

cDNA cloning and sequence analysis of odorant binding protein OBP3 in Drepanosiphum platanoidis(Homoptera:Drepanosiphidae) and testing of its adult olfactory behavior
FAN Jia,Sophie VANDERMOTEN,Frederic FRANCIS,LIU Yong,CHEN Ju-Lian,CHENG Deng-Fa.cDNA cloning and sequence analysis of odorant binding protein OBP3 in Drepanosiphum platanoidis(Homoptera:Drepanosiphidae) and testing of its adult olfactory behavior[J].Acta Entomologica Sinica,2011,54(9):975-981.
Authors:FAN Jia  Sophie VANDERMOTEN  Frederic FRANCIS  LIU Yong  CHEN Ju-Lian  CHENG Deng-Fa
Institution:FAN Jia1,2,Sophie VANDERMOTEN2,Frederic FRANCIS2,LIU Yong3,CHEN Ju-Lian1,*,CHENG Deng-Fa1,(1.State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China,2.Functional and Evolutionary Entomology,Gembloux Agro-Bio Tech,University of Liege,Passage des Déportés 2,B-5030 Gembloux,Belgium,3.College of Plant Protection,Shandong Agricultural University,Tai'an,Shandong 271018,China)
Abstract:ApisOBP3 from Acyrthosiphum pisum was known as its affinity to E-β-farnesene (EBF), and the volatile substances released by Drepanosiphum platanoidis contain no EBF which is a conventional component secreted by most of aphids. In this study, two odorant binding proteins named as DplaOBP3-1 and DplaOBP3-2 sharing high identities (95.8% and 98.32%, respectively) with ApisOBP3 were identified in D. platanoidis. The lengths of coding sequences of both DplaOBP3-1 and DplaOBP3-2 are 426 bp with 69 bp coding for signal peptide at the 5′ end. DplaOBP3-1, DplaOBP3-2 and ApisOBP3 share 6 highly conserved cysteine residues at the same position among sequences. Based on the 3D structure of LmadPBP, a pheromone binding protein from cockroach Leucophaea maderae, we modeled the structures of DplaOBP3-1 and DplaOBP3-2, and all the five hydrophobic amino acids and the aromatic amino acid residue, Tyr84, located in the binding cavity probably participating in interacting with ligands have their counterparts in the structure of ApisOBP3. Further, four-arm olfactometer assay showed that D. platanoidis were significantly repelled by both EBF and crushed aphids (P=0.01). The results suggest that volatiles from fresh crushed bodies could be the intraspecies alarm pheromone in D. platanoidis; D. platanoidis are repelled by allogenetic EBF whereas they never release EBF.
Keywords:Drepanosiphum platanoidis" target="_blank">Drepanosiphum platanoidis')" href="#">Drepanosiphum platanoidis  odorant binding protein  alarm pheromone  E-β-farnesene (EBF)  cDNA cloning  four-arm olfactometer
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