虫生真菌中对松墨天牛高毒性蛋白的筛选及性质测定

从6株虫生真菌中分离纯化了48种蛋白。以松墨天牛Monochamusalternatus为试虫，通过 生测筛选出4种高毒性蛋白，并从4种高毒性蛋白中筛选出毒力最强的蛋白Bb36W-D。蛋白Bb36W D含2个亚基，大亚基和小亚基的分子量分别约为24 kD和17 kD；等电点分别为9.47和9.32 。该蛋白含16种氨基酸，其中精氨酸、组氨酸及丙氨酸含量较高。

Screening and characterization of proteins from entomogenous fungi toxic to Monochamus alternatus ( Coleoptera : Cerambycidae)

Forty eight proteins were purified from six strains of entomogenous fungi. Four high toxic proteins were screened by bioassay, with Monochamus alternatus larvae as the targets. The further screening of 4 high toxic proteins showed that the toxicity of protein Bb36W-D was the highest in all the proteins. Protein Bb36W-D is composed of two subunits of different sizes. Molecular weight is calculated to be 24 kD for the big subunit and 17 kD for the small subunit. Isoelectric point is 9.47 for the big subunit and 9.32 for the small subunit, respectively. Protein Bb36W-D is composed of 16 ammonia acids. Concentrations of arginine, histidine and alanine are higher than others.