棉铃虫的谷胱甘肽S-转移酶(GSTs):杀虫药剂和植物次生性物质的诱导与GSTs对杀虫药剂的代谢

棉铃虫Helicoverpaarmigera（Hubner）中肠谷胱甘肽S-转移酶（GSTs）对甲基对硫磷和灭多威的代谢能力明显高于对马来酸二乙酯（DEM）和两个混剂。LD5剂量的对硫磷和灭多威对棉铃虫3龄幼虫GSTs的活性均没有诱导增加的影响，用LD50的选择剂量仅对硫磷组GSTs活性增加15％。用含0．01％的芸香苷、2-十三烷酮和槲皮素的人工饲料饲养棉铃虫经1～4代后，GSTs活性提高4～18倍。3种植物次生性物质诱导组对灭多威和溴氰菊酯的敏感度均没有明显的变化，而槲皮素组对甲基对硫磷的敏感度则降低近一半，芸香苷和2-十三烷酮组对甲基对硫磷的敏感度略有降低。这种对甲基对硫磷敏感度的变化可能与上述GSTs活性的变化有关。

GLUTATHIONE S-TRANSFERASE (GSTs) OF HELICOVERPA ARMIGERA: INDUCTION OF INSECTICIDES AND PLANT ALLELOCHEMICALS AND METABOLISM OF INSECTICIDES

Induction of glutathione S-transferases (GSTs) by insecticides and plant allelochemicals, and metabolism of insecticides by GSTs were investigated during 1992~ 1995 in cotton bollworms, Helicover pa armigera (Hubner). GSTs of the midguts from cotton bollworms metabolized parathion-methyl and methomyl more quickly than diethylmaleate, o, odiethyl-phenyl phosphorothioate (SV1 ) and mixtures of parathion-methyl and methomyl.At 24 h or 48 h after treatment with LD5 of parathion or methomyl, the GSTs activity of cotton bollworms did not increase. Selection of LD50 of parathion made GSTs activity increase 15% while selection of LD50 of methomyl had no effect on GSTs activity. Midgut GSTs activity in cotton bollworm larvae was induced up to 18-fold by feeding on the artificial diet containing 0.01 % of quercitrin compared with activity in larvae only fed with artifi-cial diet and up to 4-fold compared with activity in larvae fed with the diet containing 2-tridecanone or rutin. Toxicity of parathion-methyl to larvae of cotton bollworms reduced about 50% due to feeding on the diet with quercitrin. But the 0. 01 % of rutin, quercitrin and 2-tridecanone in diet had no effects on toxicities of methomyl and deltamethrin to larvae of cotton bollworms.