基于Cytb和ND1基因序列的弄蝶亚科(鳞翅目:弄蝶科)族级系统发育关系分析

【目的】探讨中国弄蝶亚科各族之间的系统发育关系。【方法】对22属32种弄蝶亚科蝶类的Cytb和ND1基因部分序列进行了联合分析,分别采用最大似然法(maximum likelihood,ML)和贝叶斯法(Bayesian inference,BI)构建了系统发育树。【结果】测得的Cytb和ND1基因联合后,获得的序列总长为836 bp,包含保守位点450个,变异位点386个,简约信息位点304个;A+T的平均含量为76.9%,明显高于C+G的平均含量(23.1%)。基于Cytb和ND1基因联合序列的系统发育分析显示,钩弄蝶族(Ancistroidini)、刺胫弄蝶族(Baorini)、弄蝶族(Hesperiini)为单系群,且亲缘关系较近,其亲缘关系为:(弄蝶族Hesperiini+(刺胫弄蝶族Baorini+钩弄蝶族Ancistroidini));而锷弄蝶族Aeromachini(=酣弄蝶族Halpini)是一个复系群。【结论】本文支持钩弄蝶族(Ancistroidini)、刺胫弄蝶族(Baorini)、弄蝶族(Hesperiini)的族级地位,锷弄蝶族(Aeromachini)与Warren等研究结果矛盾,其他族有待补充样本后做进一步研究。

Phylogenetic relationship of hesperiine tribes (Lepidoptera,Hesperiidae) based on Cytb and ND1 gene sequences

【Aim】 This study aims to explore and recover the tribal phylogenetic relationship within the subfamily Hesperiinae in China. 【Methods】 The partial mitochondrial Cytb and ND1 gene sequences of a total of 32 species in 22 genera were determined and analyzed, and the phylogenetic trees of the Hesperiinae species were constructed using the Bayesian and maximum likelihood methods. 【Results】 There are 450 conservative sites, 386 variable sites and 304 parsimony-informative sites, and a 76.9% A+T bias in the 836 bp nucleotides of the combined sequence of the two mitochondrial genes. Phylogenetic trees based on the combined sequence of Cytb and ND1 genes showed that the Ancistroidini, Baorini and Hesperiini are monophyletic with the relationship of (Hesperiini+(Baorini+Ancistroidini)), while the tribe Aeromachini (=Halpini) is a paraphyletic group. 【Conclusion】 Our results support the tribal nomenclatures of the Ancistroidini, Baorini and Hesperiini. However, the polyphyly of Aeromachini in this study contradicts with the former results by Warren et al. In addition, the phylogenetic relationship of other tribes needs further confirmation by more taxa sampling.