东北大黑鳃金龟嗅感器超微结构

利用扫描电镜和透射电镜对东北大黑鳃金龟Holotrichia diomphalia成虫触角嗅感器进行超微结构研究。结果表明： 其嗅感器集中于触角鳃片上，着生在表皮内陷形成的凹腔里。嗅感器包括锥形感器和板形感器两种，锥形感器根据锥体形状的差异可分为4种类型，板形感器根据盘体形状的不同可分为5种类型。嗅感器表皮为单壁，壁上具有微孔和孔道微管。嗅感器内神经元的数目并不一致，1～3个不等。雄性触角鳃片的长度长于雌性触角鳃片，并且雄性触角嗅感器的总数远远多于雌性，其中雄性板形感器的数目与雌性差异不大，但雄性锥形感器的数目却远远的多于雌性，几乎是雌性的9倍。由此推测锥形感器是感受性信息素的感器，而板形感器用于感受植物气味。

Ultrastructure of olfactory sensilla of Holotrichia diomphalia Bates ( Coleoptera: Melolonthidae)

The ultrastructure of olfactory sensilla on antennae of Holotrichia diomphalia adult was studied using SEM and TEM. The results showed that the olfactory sensilla concentrated on antennal lamellae and all these sensilla were situated on the sunken cavity formed by antennal cuticle depressions. Two types of olfactory sensillum on lamellae were found both sensilla basiconca (S. ba) and sensilla placodea (S. pl). Based on the difference of cone and plate shape, S. ba was further classified into four subtypes, and S. pl. into five subtypes. The cuticle of olfaction sensillum was single-walled, with a plenty of pores and pore tubules. The number of neuron in olfactory was inconsistent, including one olfactory receptor neuron (ORN), two ORNs and three ORNs. The length of each lamella on antenna in the male was longer than that in the female, and the total number of sensilla in the male was far more than that in the female. The number of S. pl showed no obvious difference between two sexes, but the number of S. ba in the male was far more than that in the female, about nine times that of the latter. It is so inferred that the S. ba is the sensillum used for reception of pheromone and S. pl is used for reception of plant odors.