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基于线粒体COI基因序列的大豆食心虫中国东北地理种群遗传多样性分析
引用本文:王红,徐忠新,韩岚岚,王克勤,赵奎军.基于线粒体COI基因序列的大豆食心虫中国东北地理种群遗传多样性分析[J].昆虫学报,2014,57(9):1051-1060.
作者姓名:王红  徐忠新  韩岚岚  王克勤  赵奎军
作者单位:(1. 东北农业大学农学院, 哈尔滨150030; 2. 黑龙江省农业科学院植物保护研究所, 哈尔滨150086)
摘    要:【目的】大豆食心虫Leguminivora glycinivorella (Matsumura)是一种危害大豆的主要害虫,在中国北方地区危害较重。本研究旨在探讨大豆食心虫在中国东北不同地理种群间的遗传变异。【方法】测定了10个不同地理种群153个个体的线粒体细胞色素氧化酶亚基Ⅰ (mtCOI)基因的657 bp序列,利用DnaSP 5. 0和Arlequin 3. 5. 1. 2等软件对大豆食心虫种群间的遗传多样性、基因流水平和分子变异进行分析。【结果】结果表明:10个地理种群间的COI基因共有36个变异位点和17个单倍型,其中1个单倍型为10个种群所共享。总种群的单倍型多样性指数Hd为0.456,各地理种群单倍型多样度范围在0~0.634之间。总群体的固定系数Fst为0.12545,遗传分化系数Gst为0.06326,总基因流Nm为3.49,且各种群间的基因流均大于1,种群间基因交流的水平较高。【结论】大豆食心虫种群内遗传多样性水平处于中低等水平。总群体和各种群的Tajima’s D检验结果皆不显著,说明中国东北地区大豆食心虫在较近的历史时期内没有出现种群扩张现象。AMOVA分子变异分析结果表明,大豆食心虫的遗传分化主要来自种群内部,而种群间未发生明显的遗传分化。各地理种群的单倍型在系统发育树上和中介网络图上散布在不同的分布群中,缺乏明显的地理分布格局。各种群的遗传距离与地理距离之间没有显著线性相关性,种群间的基因交流并未受到地理距离的影响。

关 键 词:大豆食心虫  线粒体COI基因  单倍型  地理种群  遗传多样性  基因流  

Analysis of the genetic diversity in geographic populations of Leguminivora glycinivorella (Lepidoptera: Olethreutidae) from northeastern China based on mitochondrial DNA COI gene sequences
WANG Hong,XU Zhong-Xin,HAN Lan-Lan,WANG Ke-Qin,ZHAO Kui-Jun.Analysis of the genetic diversity in geographic populations of Leguminivora glycinivorella (Lepidoptera: Olethreutidae) from northeastern China based on mitochondrial DNA COI gene sequences[J].Acta Entomologica Sinica,2014,57(9):1051-1060.
Authors:WANG Hong  XU Zhong-Xin  HAN Lan-Lan  WANG Ke-Qin  ZHAO Kui-Jun
Institution:(1. College of Agronomy, Northeast Agricultural University, Harbin 150030, China; 2. Institute of Plant Protection, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China)
Abstract:【Aim】 The soybean podborer, Leguminivora glycinivorella (Matsumura), is a major insect pest of soybean Glycina max, and it damages G. max seriously in northeastern China. This study aims to investigate the genetic differentiation among geographic populations of L. glycinivorella in northeastern China. 【Methods】 A 657 bp segment of the mitochondrial cytochrome c oxidase subunit I (COI) gene of 153 individuals from 10 geographic populations in northeastern China was sequenced and analyzed. The genetic diversity, gene flow and molecular variance were analyzed using DnaSP 5. 0 and Arlequin 3. 5. 1. 2.【Results】 The results showed that 36 variable sites were detected, and 17 haplotypes were defined based on the COI sequences of 10 geographic populations of L. glycinivorella, including one haplotype shared by all the 10 populations. The total haplotype diversity of all populations was 0.456, and the range of haplotype diversity of different populations was 0-0.634. The total Fst, Gst and Nm were 0.12545, 0.06326 and 3.49, respectively, and all pairwise gene flow Nm was greater than 1 in the 10 populations, suggesting that extensive gene flow occurred among the ten populations of L. glycinivorella. 【Conclusion】The L. glycinivorella populations are characterized by medium/low genetic diversity. The Tajima’s D test indicated that there might not have been a recent population expansion. Molecular variance analysis (AMOVA) demonstrated that the observed genetic differentiation occurs primarily within populations, and there is no obvious genetic differentiation among populations. The neighbor-joining phylogenetic tree and haplotype network showed that the haplotypes are distributed in different clades and no obvious geographical structure has been formed. The genetic distance is not significantly correlated with the geographical distance between populations, and the gene flow is not affected by geographical distance.
Keywords:Leguminivora glycinivorella  mtDNA COI gene  haplotype  geographic population  genetic diversity  gene flow   Leguminivora glycinivorella  mtDNA COI gene  haplotype  geographic population  genetic diversity  gene flow  
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