二斑叶螨体内感染的Wolbachia的wsp基因序列测定与分析

应用Wolbachia的wsp基因特异引物, 通过PCR扩增法检测了二斑叶螨Tetranychus urticae Koch 12个地理种群（辽宁兴城、河北昌黎、湖南长沙、河南郑州、江苏徐州、甘肃兰州、云南昆明、山东泰安、新疆石河子、宁夏银川、陕西眉县和上海）感染Wolbachia的状况。结果表明,12个地理种群全部感染了Wolbachia。克隆分离得到了它们的wsp基因序列,在GenBank的登录号依此为： AY585714、 AY585712、AY785376、AY785375、AY585713、AY785374、AY785371、AY712954、AY712955、AY785372、AY785377和AY785373。对序列进行系统发育分析,发现它们全部与B大组Ori组的Wolbachia株系十分相近或完全相同。总体来看,它们的聚类关系基本上呈平行交叉分布,在不同种群之间没有构成明显的地理分布聚集性。

Sequencing and sequence analysis of the wsp gene of Wolbachia in Tetranychus urticae Koch (Acari: Tetranychidae)

By using specific primer for wsp gene of Wolbachia and PCR method, we investigated the Wolbachia infection of twelve populations of Tetranychus urticae Koch in China. These populations were from Xingcheng in Liaoning Province, Changli in Hebei Province, Changsha in Hunan Province, Zhengzhou in Henan Province, Xuzhou in Jiangsu Province, Lanzhou in Gansu Province, Kunming in Yunnan Province, Tai’an in Shandong Province, Shihezi in Xinjiang Autonomous Region, Yinchuan in Ningxia Autonomous Region, Meixian in Shaanxi Province and Shanghai. The results showed that all the populations were infected with Wolbachia. Their sequences were submitted to GenBank and the accession numbers assigned are AY585714, AY585712, AY785376, AY785375, AY585713, AY785374, AY785371, AY712954, AY712955, AY785372, AY785377 and AY785373, respectively. By analyzing the wsp gene sequences of Wolbachia in T. urticae and constituting the phylogenetic tree, we found that they were similar to or completely identical with the wsp gene sequences of some strains of Wolbachia of Ori group in B supergroup. Since the branches of clusters are parallel, there is no evident geographic distribution clustering between different populations.