蚯蚓提取物对家蚕中肠氧化损伤、总抗氧化能力及抗氧化酶含量的影响

【目的】研究蚯蚓提取物对家蚕Bombyx mori中肠组织氧化应激及抗氧化酶含量的影响,探究蚯蚓提取物的抗氧化功能。【方法】分别给家蚕5龄幼虫饲喂蚯蚓(赤子爱胜蚓Eisenia foetida)提取液原液的50, 100和200倍稀释液处理后的桑叶,测定处理6 d后家蚕中肠组织中氧化损伤产物丙二醛(MDA)和乳酸脱氢酶(LDH)的含量;利用qRT-PCR检测家蚕中肠组织中抗氧化酶关键基因(Cat,Sod和GSH-Px)的mRNA表达水平;同时采用ELISA法检测中肠组织中抗氧化酶过氧化氢酶(CAT)、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)]含量及总抗氧化能力(T-AOC)。【结果】与对照组相比,50, 100和200倍稀释组家蚕中肠组织中MDA, CAT, SOD和GSH-Px含量及T-AOC均差异不显著,200倍稀释组LDH含量显著降低了9.85%,100倍稀释组中肠中Cat和GSH-Px的表达量均显著高于对照组,3个稀释组中Sod的表达量均显著高于对照组。【结论】添食蚯蚓提取物可通过降低家蚕中肠LDH含量、提高抗氧化酶基因的表达水平进而提高家蚕的抗氧化能力。

Effects of earthworm extract on the oxidative damage,total antioxidant capacity and antioxidant enzyme contents in the midgut of the silkworm,Bombyx mori

【Aim】 To study the effect of earthworm extract on the oxidative stress and antioxidant enzyme contents in the midgut of the silkworm, Bombyx mori, so as to explore the antioxidant function of earthworm extract. 【Methods】 The 5th instar larvae of B. mori were fed with mulberry leaves treated with the 50-, 100- and 200-fold dilutions of the original earthworm (Eisenia foetida) extract solution, respectively, the contents of malondialdehyde (MDA) and lactate dehydrogenase (LDH) as the products of oxidative damage in the midgut of the larvae treated for 6 d were determined, the expression levels of key antioxidant enzyme genes Cat, Sod and GSH-Px were assessed by quantitative real-time PCR, and the contents of antioxidant enzymes including catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and the total antioxidant capacity (T-AOC) were detected by enzyme linked immunosorbent assay. 【Results】 The contents of MDA, CAT, SOD and GSH-Px and the T-AOC in the 50-, 100- and 200-fold dilution treatment groups showed no significant difference from those in the control group, and the LDH content in the 200-fold dilution treatment group was significantly decreased by 9.85% as compared to that in the control group. The expression levels of Cat and GSH-Px in the 100-fold dilution treatment group was significantly higher than those in the control group. The expression levels of Sod in the three dilution treatment groups were significantly higher than that in the control group. 【Conclusion】 Dietary earthworm extract can improve the antioxidant capacity in B. mori by reducing the LDH content and increasing the expression level of antioxidant enzyme genes.