缺失Df(3R)Espl3/TM6C基因片段影响黑腹果蝇的睡眠时间

【目的】果蝇的睡眠活动具有生物节律性， 可受到基因的调控。为了寻找影响果蝇睡眠时间的基因， 本研究对与果蝇睡眠时间相关的基因型进行了筛选。【方法】选择黑腹果蝇Drosophila melanogaster基因缺失系5601， 8904， 7061， 7146， 27327， 669， 8103， 691， 9697， 24416， 26525， 5411， 3096， 5877和7682的7日龄成虫和野生CS品系7日龄成虫为研究对象， 利用果蝇活动监测器系统（Drosophila Activity Monitoring System， DAMS）， 记录果蝇的睡眠时间， 累计计算24 h内果蝇睡眠时间， 将测得的各品系果蝇睡眠时间进行对比分析。【结果】与野生型CS品系7日龄成虫相比， 缺失Df(3R)Espl3/TM6C基因片段的 5601品系7日龄成虫睡眠时间明显缩短（P<0.001）。【结论】缺失Df(3R)Espl3/TM6C基因片段与果蝇睡眠有关。本研究结果为揭示影响果蝇睡眠时间的基因提供数据支持， 进而为研究人类睡眠提供线索。

Deletion of gene fragment Df(3R)Espl3/TM6C affects sleep duration in Drosophila melanogaster

Aim] Sleep in Drosophila melanogaster shows biological rhythm,and it can be controlled by different genes.In order to find the proper gene that affects sleep duration,we screened the genotypes of D.melanogaster associated with sleep duration.Methods] The 7-day-old adults of the wild CS strain and the strains with gene deletion including 5601,8904,7061,7146,27327,669,8103,691,9697,24416,26525,5411,3096,5877 and 7682 of D.melanogaster were used for screening.Drosophila Activity Monitoring System(DAMS) was used to record the total sleep duration within 24 h.The data obtained were compared among different strains.Results] Compared with the wild CS strain,strain 5601 with deletion of gene fragment Df(3R)Espl3/TM6C had significantly shorter total sleep duration(P<0.001).Conclusion] The gene fragment Df(3R)Espl3/TM6C is related to the sleep duration in D.melanogaster.This research provides data support for revealing the genes which are related to sleep in D.melanogaster and more clues to studying human sleep.