微孢子虫和狄斯瓦螨分别侵染后的意蜂血淋巴蛋白质含量变化

微孢子虫Nosema apis和狄斯瓦螨微孢子虫Nosema apis和狄斯瓦螨 Varroa destructor (Acari: Varroidae)均为危害意蜂Apis mellifera的重要寄生虫，该文对其危害后意蜂血淋巴蛋白质含量的变化进行了研究。用考马斯亮蓝法测定了意蜂受侵染后血淋巴的蛋白质总量，并用高压超薄层等电点聚焦法进行血淋巴蛋白质分类。结果显示，病蜂血淋巴蛋白质总量，在人工感染微孢子虫后1～10天呈微孢子虫Nosema apis和狄斯瓦螨 Varroa destructor (Acari: Varroidae)均为危害意蜂Apis mellifera的重要寄生虫，该文对其危害后意蜂血淋巴蛋白质含量的变化进行了研究。用考马斯亮蓝法测定了意蜂受侵染后血淋巴的蛋白质总量，并用高压超薄层等电点聚焦法进行血淋巴蛋白质分类。结果显示，病蜂血淋巴蛋白质总量，在人工感染微孢子虫后1～10天呈上升趋势，然后逐渐下降，感染后12～27天保持在感染前意蜂血淋巴总蛋白质含量水平以下。螨侵染后意蜂血淋巴蛋白质含量明显增高，与健康意蜂相比差异极显著。高压超薄层等电点聚焦分析表明：狄斯瓦螨自然侵染意蜂后，意蜂血淋巴蛋白质组分与健康对照组相比发生了明显改变。这些结果提示,意蜂对于微孢子虫或狄斯瓦螨的侵染产生了一定的免疫反应。

Changes in content of hemolymph protein in the honeybee (Apis mellifera L.)workers infected by Nosema apis and Varroa destructor respectively

The changes in content of hemolymph protein in the honeybee (Apis mellifera L.) workers infected by spores of Nosema apis and the mite Varroa destructor (Acari: Varroidae) respectively were assayed. The content of hemolymph protein was detected using Bradford method. The component difference of hemolymph proteins between the healthy (CK) and the infected bees were analyzed using high voltage isoelectric focusing electrophoresis (IEF). The results showed that the content of hemolymph protein of the bees tended to rise progressively within 10 days after being infected by Nosema spores, and then decreased gradually, till reaching a level below the content before being infected in 12-27 days after infection. The content of hemolymph protein of the bees infected by V. destructor was significantly higher than that of the control, and the components of hemolymph proteins were also different between the healthy and infected bees as indicated by IEF results. The results suggested the honey bees developed immunological reaction in some degree to N. apis or V. destructor after their infection.