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内蒙古亚洲小车蝗种群遗传多样性的微卫星分析
引用本文:韩海斌,周晓榕,庞保平,张敏哲,李海平.内蒙古亚洲小车蝗种群遗传多样性的微卫星分析[J].昆虫学报,2013,56(1):79-87.
作者姓名:韩海斌  周晓榕  庞保平  张敏哲  李海平
作者单位:内蒙古农业大学农学院,呼和浩特,010019
基金项目:国家公益性行业(农业)科研专项(200903021)
摘    要: 亚洲小车蝗Oedaleus asiaticus Bei-Bienko是我国北方草原和农牧交错区主要的害虫之一。为了从分子水平评价内蒙古地区亚洲小车蝗种群的遗传多样性和种群间遗传分化, 本文采用8对微卫星引物对内蒙古15个地点的亚洲小车蝗种群进行遗传多样性分析。结果表明: 各位点有效等位基因数为3.4517~13.2881, 多态信息含量值为0.5601~0.8563, Shannon氏多样性指数在0.7018~4.1789之间。15个种群的平均期望杂合度为0.6836, Nei氏期望杂合度为0.5303~0.6513, 群体遗传距离为0.1092~0.4235, 群体分化率Fst平均值为0.1612, 基因流Nm平均值为1.6164。8个微卫星位点均具有较高的多态性, 各种群间的遗传分化水平较大, 基因交流程度中等, 个体间的遗传变异大于种群间的遗传变异。15个不同地点的亚洲小车蝗种群根据遗传距离共聚为6支。种群间遗传分化与地理距离呈正相关关系。高山和沙漠对不同地区亚洲小车蝗种群的迁移具有阻碍作用, 可能是形成遗传分化的主要原因。研究结果从分子水平探索不同地区亚洲小车蝗种群间的内在联系, 为制定亚洲小车蝗的综合治理策略提供了分子生物学的基础资料。

关 键 词:亚洲小车蝗  地理种群  遗传多样性  微卫星标记  遗传分化  聚类分析

Microsatellite marker analysis of the genetic diversity of Oedaleus asiaticus (Orthoptera: Acrididae) populations in Inner Mongolia, northern China
HAN Hai-Bin, ZHOU Xiao-Rong, PANG Bao-Ping, ZHANG Min-Zhe, LI Hai-Ping.Microsatellite marker analysis of the genetic diversity of Oedaleus asiaticus (Orthoptera: Acrididae) populations in Inner Mongolia, northern China[J].Acta Entomologica Sinica,2013,56(1):79-87.
Authors:HAN Hai-Bin  ZHOU Xiao-Rong  PANG Bao-Ping  ZHANG Min-Zhe  LI Hai-Ping
Institution:(College of Agriculture, Inner Mongolia Agricultural University, Hohhot 010019, China)
Abstract:Oedaleus asiaticus Bei-Bienko is one of the major pest insects in the grasslands and agro-pastoral ecotone in North China. By using 8 microsatellite primer pairs, the genetic diversities of 15 geographic populations of O. asiaticus in Inner Mongolia, northern China were analyzed in order to evaluate the genetic variability within populations and genetic differentiation among populations of O. asiaticus at the molecular level. The results showed that the effective number of alleles was 3.4517-13.2881, the polymorphic information content was 0.5601-0.8563 and the Shannon’s diversity index was 0.7018-4.1789. The mean expected heterozygosity of the 15 populations was 0.6836, with the Nei’s expected heterozygosity of 0.5303-0.6513 and genetic distance of 0.1092-0.4235. The mean fixation index (Fst) and the mean gene flow (Nm) were 0.1612 and 1.6164, respectively. The 8 microsatellite loci selected are of high polymorphism, this grasshopper keeps a high genetic differentiation and moderate gene flow among populations, and the genetic variability among individuals within a population is higher than that among populations. O. asiaticus populations from 15 areas were classified into 6 groups according to the genetic distance. Genetic differentiation between populations shows a positive correlation with geographical distance. High mountains and deserts always block the population migration between areas, which is possibly the main cause of genetic differentiation. The study investigates the intrinsic connection among populations from different areas at the molecular level and provides some basic information on molecular biology for developing integrated management strategies against O. asiaticus.
Keywords:Oedaleus asiaticus" target="_blank">Oedaleus asiaticus')" href="#">Oedaleus asiaticus  geographic population  genetic diversity  microsatellite marker  genetic differentiation  cluster analysis
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