抗溴氰菊酯家蝇在不同用药方式下的敏感性变化及其机制

以具有极高抗水平的抗溴氰菊酯家蝇Musca domestica vicina Macquart DR0品系为试虫，模拟田间几种常见的用药方式(混用、轮用、使用增效剂)，在室内进行平行汰选，并以不用药和继续用原药汰选的为比较，研究试虫在这几种用药方式下的敏感性变化及其变化机制。抗性家蝇用辛溴混剂、辛硫磷以及溴氰菊酯＋SV₁汰选后，在F₁₆(F₁₇)代以前，对溴氰菊酯及汰选药剂的抗性发展相对都比较缓慢；F₁₆(F₁₇)代以后，用溴氰菊酯＋SV₁汰选的家蝇对溴氰菊酯的敏感性迅速下降，抗性发展很快。家蝇对溴氰菊酯的敏感性变化与药剂中溴氰菊酯的选择压有关。生化分析结果表明，在不同用药方式汰选下，家蝇体内酯酶、多功能氧化酶、谷胱甘肽-S-转移酶、乙酰胆碱酯酶的酶活或特性发生了不同的变化。

CHANGES OF SUSCEPTIBILITY OF RESISTANT HOUSEFLY TO PESTICIDES AND IT'S MECHANISMS UNDER DIFFERENT INSECTICIDE APPLICATIONS

A housefly (Musca domestica vicina Macquart) strain (DR0) possessing high level resistance to deltamethrin was selected under different insecticide applications in the laboratory, involving alternating insecticide(phoxim), using insecticide mixture(1∶100 mixture of deltamethrin and phoxim) and deltamethrin adding synergist(SV₁). Before generation F₁₆ (F₁₇),the development of resistance to deltamethrin and selecting insecticides was relatively slow in all three strains; but after that, the rate of development increased, especially that of the deltamethrin+SV₁ selected strain to deltamethrin.The changes of susceptibility to deltamethrin were correlated with the selection pressure. Biochemical studies showed that,the changes of enzyme activities or properties of esterase, carboxylesterase, mixed-function oxidase, glutathione-S-transferase and acetylcholinesterase were responsible for the changes of susceptibility in the resistant housefly.