中华蜜蜂工蜂王浆腺活性的变化

本试验以短期体外放射化学测定法,测定了中华蜜蜂(Apis cerana cerana)工蜂的王浆腺(HP)的活性,以意大利蜂(Apis mellifera ligustica)进行对比.结果表明工蜂的HP在体外培养液中1—6小时掺入的放射性DPM几乎呈直线增加.春夏繁殖期,中华蜜蜂7日龄工蜂的HP活性最强,而意大利蜜蜂是10日龄.中华蜜蜂的越冬工蜂的HP虽呈发育状态,但活性低.而在早春,当蜂群内部出现幼虫时,越冬工蜂的HP又呈现活性.

HYPOPHARYNGEAL GLAND ACTIVITY IN THE WORKERS OF APIS CERANA CERANA

The in vitro hypopharyngeal gland (HP) activity of the workers of Apis cerana cerana (A.c.c.) of different age and at different seasons was investigated with short-term radioche-mical assay.The difference of the HP activities between A.c.c.and Apis mellifera ligustica (A.m.l.) was noticeable.The results are as follows:1.The incorporation of 3H-leucine by isolated HP and the portion secreted from the HP into the incubation medium were measured at different periods.It was seen that the rate of incorporation of 3H-lencine was almost linear over a period of 6 hours 2.During spring or summer,the activity of full-grown HP of the A.c.c workers 7 days old was the highest while in the A.m.l.workers it was highest when they were 10 days old.8.In winter,the HPs of overwintering A.c.c.workers are hy-penrophied,but the glands isolated front such bees were found to display low activity In the early spring when the colony started to breed,the HP of overwintering A.c.c,workers showed conspicuous variation in activity