| 利用RAPD对稻蝗属昆虫亲缘关系的研究 |
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| 引用本文： | 朱道弘,安藤喜,城田安.利用RAPD对稻蝗属昆虫亲缘关系的研究[J].昆虫学报,2001,44(3):316-320. |
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| 作者姓名： | 朱道弘 安藤喜 城田安 |
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| 作者单位： | 1. 中南林学院资源与环境学院;弘前大学农学院
2. 弘前大学农学院, |
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| 摘 要： | 通过20个随机引物的PCR扩增，得到了日本主要稻蝗的随机扩增多态性DNA（RAPD）图谱，根据扩增结果，计算了种间相似系数和遗传距离，建立了UPGMA系统树。结果表明，分布没有重叠、种间容易交配、能产生杂种的中华稻蝗台湾亚种与小翅稻蝗的亲缘关系最近；分布重叠的日本稻蝗与中华稻蝗台湾亚种、日本稻蝗和小翅稻蝗的亲缘关系较近。小稻蝗与其它3种稻蝗的亲缘关系较远。
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| 关 键 词： | 稻蝗 RAPD 遗传距离 亲缘关系 |
| 文章编号： | 0454-6296(2001)03-0316-05 |
Studies on the relationships among species in Oxya Serville (Orthoptera: Catantopidae) using random amplified polymorphic DNA(RAPD) |
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| ZHU Dao\|hong ,ANDO Yoshikazu ,SHIROTA Yasuyuki.Studies on the relationships among species in Oxya Serville (Orthoptera: Catantopidae) using random amplified polymorphic DNA(RAPD)[J].Acta Entomologica Sinica,2001,44(3):316-320. |
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| Authors: | ZHU Dao\|hong ANDO Yoshikazu SHIROTA Yasuyuki |
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| Institution: | ZHU Dao\|hong 1,2,ANDO Yoshikazu 2,SHIROTA Yasuyuki 2 |
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| Abstract: | Species of rice grasshoppers, Oxya japonica,O. chinensis formosana, O. yezoensis and O. hyla intricata are morphologically similar. The technique of random amplified polymorphic DNA(RAPD) was used to study their relationships. Twenty random 10\|mer primers were tested and 258 polymorphic RAPD bands were generated. A dendrogram was constructed based on genetic distance among species of rice grasshoppers by UPGMA method. It is demonstrated that the relationship between O. chinensis formosana and O. yezoensis is much close, that between O. japonica and O. chinensis formosana or O. yezoensis comes next, and the relationships between O. hyla intricata and the others are the farthest. |
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| Keywords: | rice grasshopper Oxya random amplified polymorphic DNA(RAPD) genetic distance relationship |
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