大猿叶虫四地理种群遗传多样性的RAPD分析

以大猿叶虫Colaphellus bowringi Baly 4个地理种群的基因组DNA为材料, 进行RAPD分析。从80条引物中筛选出11条稳定性好、多态性高的引物进行扩增, 共得到65个扩增位点, 53个多态位点, Nei氏遗传多样性指数为0.1049~0.2061, Shannon多样性指数为0.1641~0.3167。结果表明所分析的大猿叶虫遗传变异很高, 其中江西龙南种群遗传变异最小, 山东泰安种群遗传变异最高。种群间的遗传距离范围为0.0636~0.3200, 其中江西龙南种群和江西修水种群间的遗传距离最小, 哈尔滨种群与江西龙南种群间的遗传距离最大, 种群遗传距离的大小与其相对地理距离的远近吻合。结果提示种群遗传距离的大小与它们生物学上的相似性有关联。

Genetic diversity of four geographical populations of the cabbage beetle, Colaphellus bowringi Baly (Coleoptera: Chrysomelidae) revealed by RAPD

The genetic diversity of four geographical populations of Colaphellus bowringi Baly was analyzed by random amplified polymorphic DNA (RAPD). Sixty-five amplified loci and 53 polymorphic loci were obtained using 11 primers selected from 80 random primers. The Nei’s genetic diversity and Shannon genetic diversity indexes of the 4 populations were 0.1049-0.2061 and 0.1641-0.3167, respectively. The results showed the genetic diversity of the assayed populations was high, of which Tai’an population of Shandong (SDT) has the highest level of the genetic diversity, while Longnan population of Jiangxi (JXL) has the lowest. The genetic distances ranged from 0.0636 (between Longnan population of Jiangxi and Xiushui population of Jiangxi) to 0.3200 (between Longnan population of Jiangxi and Harbin population) among the 4 populations, indicating that the genetic distances among C. bowringi populations match with their geographical distances. The results suggest that the genetic distance might be correlated with their biological similarity.