中华蜜蜂卵表面微观结构及化学成分初步研究

为认识蜜蜂的工蜂监督机制，利用扫描电镜分别观察了中华蜜蜂Apis cerana cerana不同类型卵（蜂王产的受精卵、未受精卵以及工蜂产的未受精卵）的表面超微结构，同时用气 质联用技术测定了各类型卵新产卵（0 h）和5 h卵的表面化学信息素成分。结果表明：中华蜜蜂3种卵表面均覆有六边形的结构，结构内充满小突起，3种卵的大小及表面超微结构无显著差异。受精卵表面化学信息素种类比未受精卵更为丰富，C27∶2和C27∶1可能是卵带有的标记化学信息素。

A preliminary study on the ultrastructure and chemical components of egg surface of the Chinese honeybee, Apis cerana cerana

To understand the worker policing mechanism of honeybees, we compared the surface ultrastructure of queen-laid diploid and haploid eggs of Apis cerana cerana to that of worker-laid eggs using SEM and conducted a detailed comparative analysis on chemical components of newly laid (0 h) and 5 h eggs of various types by GC-MS. The results show that the egg surface is covered with an outer layer typified by hexagonal structures filled with small nodules and there are no differences between different types of eggs. The chemical components coating queen eggs, diploid and haploid, are more abundant than those of workers. C27∶2 and C27∶1 may be the chemical pheromones on the egg surface.