不同保藏处理的昆虫标本DNA提取及其随机扩增多态DNA反应

实验利用CTAB法对柳二十斑叶甲Chrysomelavigintipunctata (Scopoli)、异色瓢虫HarmoniaaxyridisPollas、七星瓢虫Coc cinellaseptempunctataLinnaeus、小地老虎Agrotisypsilon (Rottemberg)、红蜻CrocothemisserviliaDrury、无齿稻蝗OxyaabentataWil lemse和中华稻蝗Oxyachinensis (Thunberg)等 7种昆虫进行了基因组DNA提取。从自然干燥标本、烘干标本及酒精浸泡标本获得的DNA均可用于RAPD PCR反应 ,且烘干标本、酒精浸泡标本提取效果优于自然干燥标本。这种提取方法简便易行 ,容易掌握 ,且耗资小于其它分子生物学方法。

DNA extraction and RAPD-PCR of insect specimens preserved with different meth-ods

Genomic DNA were extracted and amplified using the polymerase chain reaction and arbitrary primers from specimens of seven insect species: Chrysomela vigintipunctata (Scopoli) (Coleptera: Chrycomelidae), Harmonia axyridis Pollas and Coccinella septempunctata Linnaeus (Coleoptera: Coccinellidae), Agrotis ypsilon (Rottemberg) (Lepidoptera: Noctuidae), Crocothemis servilia Drury (Odonata: Libellulidae), and Oxya abentata Willemse and O. chinensis (Thunberg) (Orthoptera: Catantopidae). Of the specimens assayed, some had been naturally dried, some oven dried and the remainder had been preserved in alcohol. The results suggest that, with respect to DNA extraction, oven dried specimens and those preserved in alchohol are better than naturally dried specimens. Extraction of DNA extraction using the CTAB method was easier, and more economical than other methods.