小麦几种主要次生物质对麦长管蚜几种酶活力的影响

借助麦蚜人工饲料研究明确了小麦几种主要次生物质单宁酸、总酚（没食子酸）和香豆素对麦长管蚜Sitobion avenae的存活、生长和发育有明显的抑制作用，其抗蚜阈值浓度分别为0.06%、0.08%和0.065%。用昆虫酶系体外抑制法，研究上述三种次生物质的抗蚜阈值浓度对麦长管蚜的糖转化酶和解毒酶活力的影响，结果表明: 0.06%单宁酸强烈抑制麦长管蚜蔗糖酶、海藻糖酶、羧酸酯酶和谷胱甘肽S-转移酶的活力；0.08%没食子酸显著抑制蔗糖酶、羧酸酯酶和谷胱甘肽S-转移酶的活力；0.065%香豆素极显著地抑制谷胱甘肽S-转移酶活力。

Effects of major secondary chemicals of wheat plants on enzyme activity in Sitobion avenae

The anti-herbivore effects of major secondary substances of wheat (Triticum aestivum L.), including tannic acid, total phenolic compound and coumarin, on the grain aphid, Sitobion avenae, were investigated by adding them to a chemically defined diet. The impact of these secondary chemicals on the activity of amylase, invertase, trehalase, esterase (CarE) and glutathione S-transferase (GST) in grain aphids was measured in vitro. The results show that tannic acid, total phenolic compound and coumarin significantly decreased nymphal survival, development and weight gain, with their resistant threshold concentrations being 0.06%, 0.08% and 0.065%, respectively. At these concentrations, tannic acid strongly inhibited the activity of invertase, trehalase, CarE and GST and gallic acid (total phenolic chemicals) greatly decreased the activity of invertase, CarE and GST. However, coumarin only strongly inhibited GST activity.