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入侵害虫西花蓟马及其他8种常见蓟马的分子鉴定
引用本文:游中华,路虹,张宪省,冯纪年,石宝才,宫亚军,黄大卫.入侵害虫西花蓟马及其他8种常见蓟马的分子鉴定[J].昆虫学报,2007,50(7):720-726.
作者姓名:游中华  路虹  张宪省  冯纪年  石宝才  宫亚军  黄大卫
作者单位:(山东农业大学生命科学学院,山东泰安 271018)
基金项目:国家自然科学基金委重点项目(30330090),“十一五”国家科技支撑计划(2006BAD08A08-27)
摘    要:用PCR产物直接测序法对入侵害虫西花蓟马和其他8种蓟马的线粒体 COⅠ基因433 bp片段测序,获得62个个体的序列。分子数据分析显示: 种内个体间平均遗传距离在0~0.005之间,2003年在北京发现的西花蓟马与欧洲等地区报导的西花蓟马不存在明显的遗传差异; 9种蓟马种间平均遗传距离为0.213。构建的NJ树可以很好的显示蓟马的聚类,物种各单元型最初分支自展值均达到100%。结果表明,基于PCR及直接测序技术的分子鉴定可以达到准确鉴定蓟马物种之目的。

关 键 词:缨翅目  蓟马  分子鉴定  线粒体DNA  COⅠ基因  遗传距离  NJ树  
文章编号:0454-6296(2007)07-0720-07
收稿时间:2007-01-15
修稿时间:2007-06-13

Molecular identification of the introduced western flower thrips, Frankliniella occidentalis (Pergande) and other eight common thrips species ( Thysanoptera: Thripidae)
YOU Zhong-Hua,LU Hong,ZHANG Xian-Sheng,FENG Ji-Nian,SHI Bao-Cai,GONG Ya-Jun,HUANG Da-Wei.Molecular identification of the introduced western flower thrips, Frankliniella occidentalis (Pergande) and other eight common thrips species ( Thysanoptera: Thripidae)[J].Acta Entomologica Sinica,2007,50(7):720-726.
Authors:YOU Zhong-Hua  LU Hong  ZHANG Xian-Sheng  FENG Ji-Nian  SHI Bao-Cai  GONG Ya-Jun  HUANG Da-Wei
Institution:(College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong 271018, China)
Abstract:The 433 base pair segments of the mitochondrial cytochrome oxidaseⅠ(COⅠ) gene from 62 individuals of Frankliniella occidentalis (Pergande) and other 8 common thrips species in China were sequenced. The DNA data analysis showed that the average intra-species genetic distance was 0-0.005;there was no significant genetic divergence between the western flower thrips found in Beijing in 2003 and those reported internationally. The average interspecies genetic distance was 0.213. Thrips individuals of the same species were successfully clustered by NJ tree. The bootstrap values of the original divergence among intra-species haplotypes amounted to 100%. The results show that molecular identification based on PCR and direct sequencing is desirable for identifying thrips species.
Keywords:Thysanoptera  thrips  molecular identification  mtDNA  COⅠgene  genetic distance  Neighbour-Joining tree
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