登革Ⅱ型病毒在白纹伊蚊体内分布的研究

利用蚊虫连续石蜡切片免疫组织化学技术，对登革Ⅱ型病毒(DEN-2)感染白纹伊蚊Aedes albopictus后的散播时间、程度及组织器官的感染顺序进行监测，以了解DEN-2在媒介白纹伊蚊体内的分布规律。结果表明：大剂量感染登革Ⅱ型病毒后，在蚊虫消化道的主要部位以及大多数组织器官包括神经及内分泌系统在内，如涎腺、脑、神经节等亦检测到病毒抗原。登革Ⅱ型病毒一旦感染并逸出中肠会迅速侵染其它组织。从各组织感染率的高低推断，病毒逸出中肠后通过血淋巴传播到其它组织的顺序通常为：前肠、涎腺、咽部神经节、脑及食管下神经节、后肠及复眼的小眼等。

An immunocytochemical study on the distribution of dengue 2 virus in Aedes albopictus

Dissemination of dengue 2 virus in the female adult of Aedes albopictus was studied immunocytochemically to investigate the distribution of dengue 2 virus in Ae. albopictus. The results showed that after the mosquito was infected with high-titer virus, viral antigen was detected in entire alimentary canal, especially in the midgut regions. The antigen was also detected in other tissues, including nervous and endocrine systems, such as salivary glands, brain, ganglions, etc. Sporadic viral antigen was also found in the ovarian tissues like ovarian follicle and calyces. It was inferred that once dengue 2 virus escaped from the midgut epithelium, it could quickly spread to other tissues. The most possible sequence of the virus spreading among the infected tissues was as follows: midgut, forgut, salivary glands, brain and suboesophageal ganglions, thoracic ganglions, hindgut, compound eyes and Johnston's organ.