蓖麻蚕后部丝腺体丙氨酸-tRNA和甘氨酸-tRNA同功受体相对含量的变化

用氨基酸选择性地保护相应的tRNA同功受体,使其3′端羟基不被过碘酸氧化,去氨酰化后用³²pCp标记、双向聚丙烯酰胺凝胶电泳分离的方法,分析了蓖麻蚕后部丝腺体tRNA^Ala和tRNA^Gly同功受体相对含量的变化。从5龄期24小时到85小时,这两种tRNA同功受体的数目和电泳相对位置没有明显变化,tRNA^Ala主要分为四个同功受体,tRNA^Gly主要为三个。tRNA^Ala同功受体的相对含量有变化,其中tRNA₃^Ala增加10%,tRNA₂^Ala下降11%,tRNA^Gly同功受体的相对含量无明显变化。

CHANGES OF ISOACCEPTING tRNA~(ALA) AND tRNA~(GLY) CONTENTS IN THE POSTERIOR SILKGLAND OF THE ERI-SILKWORM PHILOSAMIA CYNTHIA RICINl DURING THE FIFTH LARVAL INSTAR

The amounts of isoaccepting tRNAAla and tRNAGly in the posterior silkgland of the Eri-silkworm Philosamia cynthia ricini were analysed by selective 32P-labeling of individual species in total tRNA population. There were no evident differences in the numbers and electrophoretic behavior of fso-tRNAAla and iso-tRNAGly species from 24 hours to 85 hours during the fifth larval instar. tRNAAla was mainly divided into four species and tRNAGly three after two-dimensional polyacrylamide electrophoresis. The amounts of iso-tRNAAla changed, tRNA3Ala increasing 10% and tRNA2 Ala decreasing 11%. There were no remarkable changes in the amounts of iso-tRNAGly.