造血器官机能障碍后家蚕血淋巴中蛋白质成分的变化

为了研究家蚕Bombyx mori造血器官机能障碍后其血淋巴中蛋白质成分的变化，利用重离子射线局部照射家蚕幼虫的造血器官，检测了照射后家蚕血淋巴中的蛋白质成分及注射大肠杆菌后在体内诱导出现的应急蛋白量的变化。结果表明，照射蚕血淋巴中的蛋白质含量与对照蚕之间没有明显的差异。但在成分分析时发现，5龄起蚕血淋巴中70 kD附近的3条蛋白质谱带比对照蚕的浓度要高，随着个体的发育两者的浓度都上升；5龄后期则相反，对照蚕的浓度比照射蚕高；脂肪体中贮藏蛋白质的含量具有相似的变化趋势。用家蚕贮藏蛋白质SP-1及SP-2的抗血清进行免疫印迹反应的结果显示：70 kD附近的3条蛋白质谱带的最上面的一条为贮藏蛋白质SP-1，下面的二条为贮藏蛋白质SP-2；同时照射蚕血淋巴中分子量约为24 kD的蛋白质成分也发生变化，5龄前期的浓度比对照蚕低，5龄第3天几乎检测不到；全体照射与造血器官局部照射蚕之间的结果相似。照射蚕注射大肠杆菌后在体内诱导出现的应急蛋白量明显比对照蚕要少。由此认为家蚕幼虫造血器官与血淋巴中的蛋白质成分有关，造血器官的机能障碍、血球的数量减少可影响脂肪体中蛋白质的合成，从而使存在于血淋巴中的蛋白质成分发生变化。

Change in hemolymph proteins of silkworm after hemopoietic organs being damaged

To study the change of proteins in hemolymph after hemopoietic organs of silkworm (Bombyx mori) being damaged, heavy ion beams (¹²C⁵⁺, 18.3 MeV/μ, range = 1.1 mm, 100 Gy) were used to locally irradiate the hemopoietic organs-wing disc complex at the premolting stage of the 4th instar. The total hemolymph proteins and bacterial challenge-related proteins induced by E. coli were investigated during the 5th instar. The results indicated that there was no significant difference in hemolymph proteins between irradiated individuals and the controls. Only the concentration of 70 kD proteins in irradiated silkworms were observed higher at day 0 of the 5th instar and lower at the later stage of the 5th instar than those of the respective controls. The content of storage protein in fat body showed the similar change tendency with that in hemolymph. The storage proteins were analyzed by Western blotting, and the results showed the 1st protein band near 70 kD was storage protein SP-1, the below two were storage proteins SP-2. Additionally 24 kD proteins in irradiated individuals also changed: it was lower than that of the controls at early days, hardly detected at the 3rd day of 5^th instar. Similar effects were also observed in the larvae generally irradiated with heavy ion beams. For irradiated individuals the quantity of bacterial challenge-related proteins in hemolymph induced by E. coli significantly decreased. It was so concluded that silkworm hemopoietic organs were related to the formation of hemolymph proteins, and due to the decrease of hemocytes caused by functional disruption of hemopoietic organs, synthesis of protein in fat body was affected and protein components in hemolymph changed.