| 昆虫细胞系的培养和建立技术 |
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| 引用本文： | 张寰,张永安,秦启联,王玉珠,曲良建,李瑄,苗麟,殷珍仙,张爱君,温发园.昆虫细胞系的培养和建立技术[J].昆虫学报,2007,50(8):834-839. |
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| 作者姓名： | 张寰 张永安 秦启联 王玉珠 曲良建 李瑄 苗麟 殷珍仙 张爱君 温发园 |
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| 作者单位： | (中国科学院动物研究所，农业虫害鼠害综合治理研究国家重点实验室，北京 100080) |
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| 基金项目： | 国家“948”项目(2005-4-30)，科技部农业科技成果转化资金项目(04EFN216700334) |
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| 摘 要： | 迄今已经报道的昆虫细胞系有800株以上。昆虫细胞系在昆虫病理学、寄生虫学、内分泌学、遗传学和分子生物学等基础和应用研究中得到越来越广泛的应用。本文结合我们研究的结果和实践经验，概括了国内外昆虫细胞系建立技术的研究进展，包括昆虫细胞培养的发展、昆虫细胞系建立技术、不同昆虫组织来源细胞系的建立方法和过程，以及对昆虫细胞系特征的鉴定等方面。
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| 关 键 词： | 昆虫 细胞培养 细胞系 建立技术 |
| 文章编号： | 0454-6296（2007）08-0834-06 |
| 收稿时间： | 2007-02-15 |
| 修稿时间： | 2007-05-11 |
Advances in establishment of insect cell lines |
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| ZHANG Huan,ZHANG Yong-An,QIN Qi-Lian,WANG Yu-Zhu,QU Liang-Jian,LI Xuan,MIAO Lin,YIN Zhen-Xian,ZHANG Ai-Jun,WEN Fa-Yuan.Advances in establishment of insect cell lines[J].Acta Entomologica Sinica,2007,50(8):834-839. |
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| Authors: | ZHANG Huan ZHANG Yong-An QIN Qi-Lian WANG Yu-Zhu QU Liang-Jian LI Xuan MIAO Lin YIN Zhen-Xian ZHANG Ai-Jun WEN Fa-Yuan |
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| Institution: | (State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China) |
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| Abstract: | More than 800 insect cell lines have been reported by now. Insect cell lines have become useful tools with a variety of applications in such research fields as insect pathology, parasitology, endocrinology, genetics, molecular biology, etc. This paper reviews the progresses in the technique of establishing insect cell lines in combination with our results and experiences gained in recent years, which include development of insect culture, establishment of insect cell lines, initiation of a cell line from different tissues, characterization and identification of new insect cell line. |
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| Keywords: | Insect cell culture cell line establishment |
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