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四纹豆象不同地理种群的遗传分化
引用本文:顾杰,毛雅琴,王莉萍,许佳君,张愚,杜予州.四纹豆象不同地理种群的遗传分化[J].昆虫学报,2009,52(12):1349-1355.
作者姓名:顾杰  毛雅琴  王莉萍  许佳君  张愚  杜予州
作者单位:1. 扬州大学应用昆虫研究所,江苏扬州,225009;江苏出入境检验检疫局,南京,210001
2. 扬州大学应用昆虫研究所,江苏扬州,225009
3. 扬州大学应用昆虫研究所,江苏扬州,225009;扬州出入境检验检疫局,江苏扬州,225009
基金项目:江苏出入境检验检疫局科技项目,江苏省科技攻关项目 
摘    要:【目的】通过对四纹豆象Callosobruchus maculatus Fabricius不同地理种群mtDNA-Cytb和COⅠ基因部分序列进行比较, 分析其不同地理种群间的遗传分化情况, 为揭示其与生物入侵的关系及入侵过程中种群系统发育地理格局与演变机制提供依据。【方法】用PCR产物直接测序法对分别来自中国海南、喀麦隆、韩国和泰国的四纹豆象4个地理种群的mtDNA-Cytb和COⅠ序列进行测序, 运用软件MEGA3.1对四纹豆象不同地理种群mtDNA-Cytb和COⅠ序列进行序列分析, 以绿豆象C. chinensis为外群构建了不同单倍型的分子系统树。【结果】34条420 bp Cytb序列中共检测到14个多态位点和5种单倍型, 33条822 bp COⅠ序列中检测到28个多态位点和9种单倍型, 其中4种单倍型为独享单倍型, 其余为全部或部分种群的共享单倍型。AMOVA分析结果显示, 四纹豆象4个地理种群间的遗传结构差异并不明显, 遗传差异主要发生在地理种群内。对4个地理种群进行了Fst值和基因流动统计, 结果表明4个地理种群间既存在着一定数量的基因交流, 也存在一定程度的遗传分化。【结论】根据单倍型分布格局初步推测, 中国不可能是四纹豆象的原产地, 而喀麦隆有可能是原产地之一, 并且喀麦隆种群与泰国种群之间的基因交流比较充分, 而中国种群与其他种群之间的遗传分化相对较大。

关 键 词:四纹豆象  mtDNA-Cytb    mtDNA-COⅠ  单倍型  地理种群  遗传分化  

Genetic differentiation among different geographic populations of the cowpea weevil, Callosobruchus maculatus (Coleoptera: Bruchidae)
GU Jie,MAO Ya-Qing,WANG Li-Ping,XU Jia-Jun,ZHANG Yu,DU Yu-Zhou.Genetic differentiation among different geographic populations of the cowpea weevil, Callosobruchus maculatus (Coleoptera: Bruchidae)[J].Acta Entomologica Sinica,2009,52(12):1349-1355.
Authors:GU Jie  MAO Ya-Qing  WANG Li-Ping  XU Jia-Jun  ZHANG Yu  DU Yu-Zhou
Abstract:Objective] Through comparing partial sequences of mitochondrial Cytb and COI genes, to analyze the genetic differentiation among the geographic populations of the cowpea weevil, Callosobruchus maculatus Fabricius, and to determine the relationships between the genetic structure of populations and biological invasion and provide an understanding of the evolutionary mechanisms underlying the invasion process. Methods] The mtDNA-Cytb and COI regions of 4 populations of C. maculatus from Hainan of China, Cameroom, Korea and Thailand, respectively, were sequenced by direct sequencing method. The mtDNA Cytb and CO I sequences among populations of C. maculatus were analyzed using MEG A3. 1 and the phylogenetic trees of all haplotypes were constructed using C. chinensis as the out-group. Results] As for the 420 bp mtDNA-Cytb gene of 34 individuals, 14 polymorphic sites and 5 haplotypes were found, while as for the 822 bp mtDNA-Cytb gene of 33 individuals, 28 polymorphic sites and 9 haplotypes were found. Four haplotypes were rare haplotypes, typically present in only one or two individuals and representative of a specific population, while other haplotypes were shared by all or partial populations. Molecular variance analysis (AMOVA) demonstrated that the genetic variation was not obvious and mainly occurred within geographic populations. The Fst value and the Nm value showed that not only the gene flow occurred among the four populations, but also the genetic differences did. Conclusion] Given the observed distribution pattern of haplotypes, China was not the origin of C. maculatus, while Cameroon was likely to be one of the origins of C. maculatus. Gene flow between Haplotype Cameroon and Haplotype Thailand was relatively abundant, and genetic differentiation among Haplotype China and other haplotypes were relatively large.
Keywords:mtDNA-Cytb  Callosobruchus maculatus  mtDNA-Cytb  mtDNA-CO I  haplotype  geographic population  genetic differentiation
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